上海农业学报2012，Vol.28Issue(1)：17-21,5.
牡丹组织培养若干影响因子研究
Several factors influencing tissue culture of Paeonia suffruticosa
摘要
Abstract
The peony cultivar 'Taipinghong' was used as a test material,and tissue culture of peony was studied under using different sterilization methods,anti-browning materials,hormone recipes, media and rooting conditions. The result showed that the effect of sterilizing with 0. 1% mercuric chloride solution for 8 min and then with 7% sodium hypochlorite solution for 8 min was the best,in which the contamination rate was zero for the scaly buds,4.5% for the underground buds and 22.0% for the seeds; Adding 0.5 g/L PVPP and 0.5 g/L AC in modified MS medium could effectively mitigate browning of the peony explants; Modified MS + 1. 0 mg/L BA + 0. 2 mg/L NAA +0.3 mg/L GA + 0. 5 g/L CH + 0.5 g/L PVPP +0.5 g/L AC was suitable for the scaly bud induction; MS + 1. 5 mg/L BA + 0.2 mg/L NAA + 1.0 g/L PVPP was suitable for the peony bud propagation; And 1/2 MS + 5.0 mg/L IBA + 0.5 g/L AC was suitable for the test-tube seedling rooting.关键词
牡丹/组织培养/增殖/褐化/生根Key words
Paeon ia suffruticosa/Tissue culture/propagation/Browning/Rooting分类
农业科技引用本文复制引用
殷丽青,王新其,胡永红,刘炤,李秀芬,张建军..牡丹组织培养若干影响因子研究[J].上海农业学报,2012,28(1):17-21,5.基金项目
上海市科技兴农重点攻关项目(沪农科攻字2008第10-4号) （沪农科攻字2008第10-4号）
上海市重点科技攻关项目(10391901200,10dz2253700)资助 （10391901200,10dz2253700）
