同济大学学报（医学版）2012，Vol.33Issue(1)：1-4,12,5.DOI:10.3969/j.issn1008-0392.2012.01.001
模拟肺移植后闭塞性细支气管炎小鼠模型的建立
A murine model of obliterative bronchiolitis after lung transplantation
摘要
Abstract
Objective To establish a murine model of obliterative bronchiolitis (OB) after lung transplantation. Methods Pathogen-free male C57BL /6 ( n = 25 ) and BALB / c ( n = 5 ) mice weighing 20 ~ 25 g were used for experiments. Weight-matched mice were assigned to two groups for orthotopic tracheal transplant. Experimental group: the trachea from BALB / c ( n = 5 ) was transplanted to C57BL /6 (n - 10); Control group: the trachea from C57BL /6 ( n = 5 ) was transplanted to C57BL /6 (n = 10) mice. Native and transplanted lungs were harvested, stained with H&E, and examined under light microscopy. Results The mortality was 0% (0/20) during the process of model establishment and four-week mortality was 5% (1/20). Pathology revealed the trachea endothelial cells in experimental group were damaged, accompanied by enhanced inflammatory cell infiltration and fibro hyperplasia. Incidence of obliterative bronchiolitis in mice of experimental group was approximately 100% , but control group showed no pathological changes in lung tissue. Conclusion Murine trachael transplantation model has been established successfully may be used to study the mechanism of OB.关键词
气管移植/肺移植/闭塞性细支气管炎/动物模型/小鼠Key words
tracheal transplantation/ lung transplantation/ obliterative bronchiolitis animal model/ mice分类
医药卫生引用本文复制引用
王建刚,史乾,曹浩,周晓慧,范慧敏..模拟肺移植后闭塞性细支气管炎小鼠模型的建立[J].同济大学学报（医学版）,2012,33(1):1-4,12,5.基金项目
国家自然科学基金(81070073) （81070073）
上海市科委(09411962200) （09411962200）
浦东新区卫生系统优秀青年医学人才培养计划(PWRq201006) （PWRq201006）
浦东新区卫生系统重点学科群建设(PKzxkq2010-01) （PKzxkq2010-01）
