生命科学研究2012，Vol.16Issue(1)：48-53,6.
小鼠肝癌及肝正常组织B4GalT糖基转移酶家族mRNA表达差异及其对细胞膜相关糖链的影响
Different Expression of mRNA in B4GalT Glycosyltransferase Family Between Mouse Hepatocellular Carcinoma and Normal Liver Tissues and its Influence on Relevant Sugar Chain of Membrane
摘要
Abstract
To investigate different expression of mRNA in B4GalT (β-1, 4-Galactosyltransferase) family between mouse hepatocellular carcinoma and normal liver tissues and its influence on relevant sugar chain of membrane. The different expression of mRNAs in 7 members of B4GalT family and α-2, 3 sialyltransferase ST3Gal I, ST3Gal IV, ST3Gal VI and fucosyltransferase FUT8 were detected with RT-PCR. The expression levels of galactose, fucose and sialic acid on cell membrane surface were analyzed by lectin array. The results showed that compared with normal mouse tissues, B4GalT-l, B4GalT-3, ST3Gal IV, ST3Gal VI and FUT8 in mouse hepatocellular carcinoma were identified highly expressed. Galactose, fucose and sialic acid on cell membrane surface were increased, indicating that B4GalT-l and B4GalT-3 are related with the increasing of the galactose epitope. Because sialic acids were added to Galβ-1, 4-GlcNAc epitopes to form slewis x antigen precursor by ST3Gal Ⅲ, ST3Gal IV or ST3Gal VI, the relationship between the mRNA expression ofB4GalT-l and B4GalT-3 with ST3gGal IV, ST3Gal V and FUT8 in the experiment indicates that B4GalT-l and B4GalT3 may effect synergistically with ST3Gal IV, ST3Gal VI and FUT4, which results in the increase of galactose, fucose and sialic acid on hepatocellular carcinoma cell membrane.关键词
B4GalT/肝癌/细胞膜/糖基化Key words
B4GalT/liver cancer/cell membrane/glycosylation分类
医药卫生引用本文复制引用
马汝海,王冬青,潘忠诚,王天骄,何群,赵雨杰..小鼠肝癌及肝正常组织B4GalT糖基转移酶家族mRNA表达差异及其对细胞膜相关糖链的影响[J].生命科学研究,2012,16(1):48-53,6.基金项目
国家自然科学基金资助项目(20672144) （20672144）
