生命科学研究2012，Vol.16Issue(1)：59-62,4.
体外构建组织工程化软骨的初步研究
A Primary Study on Constructing Tissue Engineering Cartilage In vitro
摘要
Abstract
Observing the growth condition of cartilage after the long culturing with calcium alginate gels in vitro, in order to provide a primary study on constructing tissue engineering cartilage in vitro with calcium alginate gels. Subculturing cartilage cells mixed with alginate gels to create a final cell concentration of 1×107/mL. Then pouring in cylindrical tooting , with different concentration of calcium solution 100, 200, 300 mmol/L was added separately, cured and solidified for 15 minutes to shape. After 2, 4, 6 weeks culturing in vitro, HE and Alcian blue staining were made to know the growth condition of cartilage cells. By culturing , cartilage cells can abundantly amplify, and hold activity and proliferation in the concentration of 100, 200 and 300 mmol/L calcium alginate gels. At 6 weeks, appearing resemble cartilage. So alginate gels is a relatively suitable biomaterial for constructing tissue engineering cartilage in vitro.关键词
组织工程/软骨细胞/藻酸盐Key words
tissue engineering/cartilage/alginate分类
医药卫生引用本文复制引用
王晋平,赵大庆..体外构建组织工程化软骨的初步研究[J].生命科学研究,2012,16(1):59-62,4.基金项目
国家自然科学基金资助项目(30772261) （30772261）
