色谱2012，Vol.30Issue(1)：91-94,4.DOI:10.3724/SP.J.1123.2011.07014
QuEChERS-高效液相色谱-串联质谱法测定黄瓜和土壤中甲基硫菌灵和多菌灵
Determination of thiophanate-methyl and carbendazim in cucumber and soft by QuEChERS-high performance liquid chromatography-triple quadrupole tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of thiophanate-methyl and carbenda-zim residues in cucumber and soil by using QuEChERS-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). The residues in the samples were extracted by acetonitrile, cleaned up by developed QuEChERS method , and then analyzed by using LC-MS/MS in multiple reaction monitoring (MRM) mode via positive electrospray ionization with an Agilent ZORBAX SB-C18 column (30 mm x 2. 1 mm, 5 ixm) as the analytical column. The recoveries of thiophanate-methyl spiked at four levels of 0. 01, 0.05, 0. 1 and 1. 0 mg/kg were from 87. 3% to 96. 0% with the relative standard deviations (RSDs) of 8. 0% -9. 3% in cucumber, from 88. 8% to 93. 4% with the RSDs of 5. 3% -9. 9% in soil; the recoveries of carbendazim spiked at the same levels as those of thiophanate-methyl were from 87. 1% to 92. 3% with the RSDs of 5. 2% -7. 5% in cucumber, from 85. 8% to 90. 9% with the RSDs of 5. 3% - 13. 2% in soil. The method is simple, rapid and characterized with acceptable sensitivity and accuracy to meet the requirements of the pesticide residue analysis. This method is applicable to confirm the residues of thiophanate-methyl and carbendazimin cucumber and soil.关键词
QuEChERS/高效液相色谱-串联质谱/甲基硫菌灵/多菌灵/黄瓜/土壤Key words
QuEChERS/ high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) / thiophanate-methyl/ carbendazini/ cucumber/ soil分类
化学化工引用本文复制引用
张志勇,龚勇,单炜力,简秋,沈燕,刘贤进..QuEChERS-高效液相色谱-串联质谱法测定黄瓜和土壤中甲基硫菌灵和多菌灵[J].色谱,2012,30(1):91-94,4.基金项目
江苏省农业科技自主创新基金项目(CX(11)4069). （CX(11）
