色谱2012，Vol.30Issue(2)：170-177,8.DOI:10.3724/SP.J.1123.2011.11020
依赖色谱保留时间的智能化选择反应监测质谱方法的建立及其初步应用
Establishment of retention time-dependent intelligent selected reaction monitoring mass spectrometric method and its application
摘要
Abstract
A method of liquid chromatographic retention time-dependent intelligent selected reaction monitoring mass spectrometry (iSRM MS) was established for the identification of targeted proteome, and was compared in detail with the method without liquid chromatographic retention time-dependent iSRM MS in the analysis results of different samples, such as the pep-tide mixtures from bovine serum albumin, six standard proteins or Thermoanaerobacter teng-congensis extract digested by trypsin. The results showed that the throughput of the identified peptides and the proteins was evidently increased with the method of liquid chromatographic retention time-dependent iSRM MS, especially in the complex sample such as Thermoanaerobacter tengcongensis extract. The coverage of the identified peptides and proteins from Thermoanaerobacter tengcongensis extract was reached 89. 62% and 92. 41% of the number of targeted peptides and proteins respectively. Higher sensitivity and reproducibility were also obtained. In addition, peptides with the same m/z but different retention times could be identified accurately when the method was used. With the higher throughput, better sensitivity and excellent reproducibility, the method of liquid chromatographic retention time-dependent iSRM MScan play an important role in the identification of targeted proteomes of complex biological samples in the future.关键词
液相色谱/保留时间/智能化选择反应监测/质谱/目标蛋白质组学/腾冲嗜热菌Key words
liquid chromatography (LC)/retention time/intelligent selected reaction monito-ring (iSRM)/mass spectrometry ( MS)/targeted proteomics/Thermoanaerobacter tengcon-gensis分类
化学化工引用本文复制引用
毛心丽,卫军营,牛明,周廉淇,王雪颖,佟巍,秦伟捷,张养军,钱小红..依赖色谱保留时间的智能化选择反应监测质谱方法的建立及其初步应用[J].色谱,2012,30(2):170-177,8.基金项目
国家重大科学计划项目(2012 CB910603和2010CB912701)和国家自然科学基金项目(20735005、20875101和20905077). （2012 CB910603和2010CB912701）
