色谱2012，Vol.30Issue(2)：184-189,6.DOI:10.3724/SP.J.1123.2011.10037
柱前衍生高效液相色谱法测定茶叶中茶氨酸及y-氨基丁酸
Determination of theanine and γ-aminobutyric acid in tea by high performance liquid chromatography with precolumn derivatization
摘要
Abstract
A method of precolumn derivatization-high performance liquid chromatography(HPLC)for the determination of theanine and γ-aminobutyric acid(GABA)in tea was established. O-Phthalaldehyde(OPA)and N-acetyl-L-cysteine(NAC)were chosen as the derivatization reagents. The effects of teapolyphenol(Tp),proline(Pro)and Vitamin C(Vc)on derivatization yields were investigated. The results indicated that Vc not only stabilized the stock solution of OPA, but also enhanced the yield of GABA derivative. However, the yield of theanine derivative was less affected. The HPLC separation system was also optimized. The resolution of the derivatives was improved by adjusting the pH value and phosphate-citric buffer concentration of the mobile phase. The limits of detection(LODs)for GABA and theanine were 3.01 × 10-5 mmol/L and 7.98 × 10-5 mmol/L, and the limits of quantification(LOQs)were 9. 99 × 10-5 mmol/L and 2. 658 × 10-4 mmol/L, respectively. The linear ranges of GABA and theanine were 0.01 -0.4 mmol/L with the correlation coefficient of 0. 996 and 0.05 - 0. 8 mmol/L with the correlation coefficient of 0. 995, respectively. The main recoveries for GABA and theanine in green tea, Oolong tea, and black tea, ranged from 99.29％ to 119.60％ and from 62. 88％ to 141.06％ respectively. The method with simple procedure and efficient separation was proved to be suitable for the determination of GABA and theanine in tea.关键词
高效液相色谱/柱前衍生/γ-氨基丁酸/茶氨酸/茶/邻苯二甲醛/N-乙酰-L-半胱氨酸Key words
high performance liquid chromatography ( HPLC )/precolumn derivatization/γ-aminobutyric acid/theanine/tea/o-phthalaldehyde/N-acetyl-L-cysteine分类
化学化工引用本文复制引用
涂云飞,杨秀芳,张士康,朱跃进..柱前衍生高效液相色谱法测定茶叶中茶氨酸及y-氨基丁酸[J].色谱,2012,30(2):184-189,6.基金项目
This work was partly supported by Zhejiang Provincial Natural Science Foundation of China (No.Y3100683). （No.Y3100683）
