中国生态农业学报2012，Vol.20Issue(1)：40-47,8.DOI:10.3724/SP.J.1011.2012.00040
灌浆结实期高温干旱复合胁迫对稻米直链淀粉及蛋白质含量的影响
Effects of combined high temperature and drought stress on amylose and protein contents at rice grain-filling stage
摘要
Abstract
Grain-filling stage is a critical stage for rice yield and quality.There have been frequent high temperatures and droughts in several rice production regions in South China due mainly to global climate change but also to local topographic conditions.This has resulted in simultaneous decline in rice yield and quality.This study investigated the combined effects of high temperature and drought stress on rice quality at grain-filling stage via analyzing the changes in amylose and protein contents of indica rice (intro-gression line of H5 with induced genes of Alternanthera hiloxeroides).A pot experiment was conducted in greenhouse conditionswith experimental treatments including optimal temperature plus moderate drought (OT-MD),optimal temperature plus severe drought (OT-SD),high temperature plus well-watered condition (HT-WW),high temperature plus moderate drought (HT-MD),high temperature plus severe drought (HT-SD) and then optimal temperature plus well-watered condition (OT-WW) as the control treatment (CK).In addition to CK,treatment durations were set at 10 days,20 days,30 days and 40 days,respectively.The results showed that while amylose content decreased,that of protein increased during high temperature period.Compared with CK,amylose content dropped by 1.783%～5.987% while that of protein increased by 1.178%～2.741% during 10～40 days heat period.A similartrend was noted for drought conditions.In other words,amylose content decreased while protein content increased during drought.Compared with CK,amylose content decreased by 1.956%～6.270% while protein content increased by 1.153%～2.944% under 10～40 days of OT-MD treatment.Stress-driven changes in amylose and protein contents were higher under OT-SD treatment than under OT-MD treatment.While combined drought and high temperature stress reduced amylose content,it enhanced protein content.The ranges for amylose and protein contents of rice due to combined high temperature and drought stress were higher than that due to theindividual high temperature or drought stress.The degree of changes in amylose and protein contents increased with increasing intensity,duration and frequency of the stress.Rice quality deterioration at the 20th days of grain-filling was mainly caused by the effect of combined high temperature and drought stress.An ascending order of the stress effect arrangement on amylose and protein contents under the same treatment periods was as follows: HT-WW < OT-MD < OT-SD < HT-MD < HT-SD.This study laid the basis for drought and heat resistance of rice,a critical element in rice breeding and cultivation.关键词
水稻/高温胁迫/干旱胁迫/复合胁迫/直链淀粉/蛋白质/灌浆结实期/胁迫效应Key words
Rice (Oryza sativa L.)/High temperature stress/Drought stress/Combined stress/Amylose/Protein/Grain-filling stage/Stress effect分类
农业科技引用本文复制引用
高焕晔,王三根,宗学凤,腾中华,赵芳明,刘照..灌浆结实期高温干旱复合胁迫对稻米直链淀粉及蛋白质含量的影响[J].中国生态农业学报,2012,20(1):40-47,8.基金项目
重庆市自然科学基金重点项目(CSTC,2009BA 1006)、重庆市水稻良种创新重大专项(CSTC,2007AB1033)、重庆市科技攻关计划项目(CSTC,2007AC 1051)、贵州省科技重大专项计划项目(黔科合重大专项字[2011]6012-1号)资助 （CSTC,2009BA 1006）
