中国生态农业学报2012，Vol.20Issue(3)：343-347,5.DOI:10.3724/SP.J.1011.2012.00343
高温强光下Ca2+对西葫芦幼苗膜质过氧化、抗氧化酶系统及热耗散的影响
Effects of Ca2+ on membrane lipid peroxide, antioxidation enzymes and thermal dissipation of squash seedlings under high temperature and light intensity
摘要
Abstract
Vegetable growth and development have suffered restraints and damages by high temperatures and strong lights under rising global temperatures in recent years. The yield, quality and economic benefits of summer vegetables substantially reduced due to dual stresses of high temperatures and strong lights. Therefore an experiment was carried out in 2009 to explore the methods of alleviating of high temperature/light adversities in vegetable production. In the experiment, a squash (Cucurbitapepo L.) variety of "Alan" was used to study Ca2+ effects on the characteristics of chlorophyll fluorescence, membrane lipid peroxide and antioxidation enzymes of squash seedlings by exogenous application of CaCb solution under the stresses of high temperature and light intensity. The results showed that squash seedlings under the application of 5~20 mmol-L"1 of Ca2+had higher superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and catalase (CAT) activities and GSH content than the control experiment (CK). However, the contents of malondialdehyde (MDA), H2O2, O2t and relative electrical conductivity were lower than those of over-40 mrnol-L-1 Ca2+and CK treatments. The maximum PSII efficiency (Fv/Fm), actual PSII efficiency (0PS E) and photochemical queching coefficient (qP) improved under the 5~20 mmol-L-1 Ca2+ treatment. However, non-phochemical fluorescence quenching coefficient (NPQ) decreased under the 5-20 mmol-L-1 Ca2+ treatment. This indicated that the 5~20 mmol-L-1 Ca2+ treatment remarkably buffered damages caused by high temperatures and light intensity stresses and decreased excitation energy of dissipation. The best buffer effect for high temperatures and strong lights occurred under 10 mmol-L-1 Ca2+ application. No buffer effect was noted when Ca2+ concentration was more than 40 mmol-L-1.关键词
西葫芦/高温强光/Ca2+处理/保护酶活性/膜脂过氧化/热耗散Key words
Squash/High temperature and light intensity/Ca2+ treatment/Protective enzyme/Membrane lipid peroxide/Thermal dissipation分类
农业科技引用本文复制引用
秦舒浩,张俊莲,孔令娟,张文莉..高温强光下Ca2+对西葫芦幼苗膜质过氧化、抗氧化酶系统及热耗散的影响[J].中国生态农业学报,2012,20(3):343-347,5.基金项目
甘肃省科技支撑计划项目(1104NKCA084)、甘肃省自然科学基金项目(096RJZA009)和兰州市科技攻关项目资助 （1104NKCA084）
