生态学杂志2012，Vol.31Issue(4)：830-836,7.
利用AFLP和SSAP研究短芒大麦突变系种群遗传多样性
Population genetic diversity of Hordeum brevisubulatium ( Trin.) Link mutation lines based on AFLP and SSAP analyses
摘要
Abstract
By using amplified fragment length polymorphism (AFLP) and specific-sequence amplified polymorphism (SSAP) markers, this paper studied the genetic diversity and genetic structure of four Hordeum brevisubulatum (Trin. ) Link populations mainly comprised of salt-resistant mutants from the Songnen Plain of Northeast China. Due to the slight difference of SSAP and AFLP markers in their principles, there existed small differentiation in the analytical results, but the results had a high level correlation by Mantel test ( r = 0. 88 , P<0. 01). The AFLP and SSAP analyses showed that the total proportion of polymorphic loci (P) was 23. 3% and 37. 7% , Shannon index was 0. 10 and 0. 14, Nei' s gene diversity index was 0. 07 and 0. 09, and gene differentiation coefficient ( Gst) was 0. 49 and 0. 44, respectively. These results indicated that there was a high level genetic diversity within the four populations, though they all formed separately from a mutant line. The AMOVA and cluster analyses also indicated that the population genetic differentiation mainly existed within populations, though there was a high level genetic difference among the populations.关键词
短芒大麦/遗传结构/遗传变异/AFLP/SSAPKey words
Hordeum brevisubulatium (Trin. ) Link/ genetic structure/ genetic differentiation/ AFLP/ SSAP.分类
农业科技引用本文复制引用
单晓辉,李毅丹,李彦舫,原亚萍..利用AFLP和SSAP研究短芒大麦突变系种群遗传多样性[J].生态学杂志,2012,31(4):830-836,7.基金项目
吉林大学农学部博士科研启动基金项目(4305050102B1)资助. （4305050102B1）
