生态学杂志2012，Vol.31Issue(4)：1000-1008,9.
耐盐碱菌株的分离筛选及生物学特性和盐碱去除效率的研究
Isolation and screening of saline-alkali tolerant bacterial strains and the biological characteristics and salt-alkali removal efficiency of the strain screened
燕红 1钟方 1高新亮 1刘蕊1
作者信息
- 1. 哈尔滨理工大学化学与环境工程学院,黑龙江省高校绿色化工技术重点实验室,哈尔滨市150040
- 折叠
摘要
Abstract
The soil samples collected from a saline-alkali land in Daqing City of Heilongjiang Province, Northeast China were properly diluted, and smeared on a high-alkali culture medium. Forty one bacterial strains were isolated and purified. Further screening of the strains by progressively increasing the salt and alkali concentrations in the culture medium respectively or simultaneously resulted in the discovery of a saline-alkali tolerant strain which could even grow in a medium with pH 13 and containing 200 g ? L"1 of NaCl. The strain belonged to both extreme halo-phile and extreme alkaliphile. The study of the biological characteristics and salt-alkali removal efficiency of the strain under different culture conditions including carbon source, nitrogen source, carbon/nitrogen ( C/N) ratio, initial pH value, and temperature indicated that for the growth and the salt-alkali removal efficiency of the strain, beef extract was the best carbon source, peptone was the best nitrogen source, and the optimum C/N ratio was 4:1. For the growth of the strain, the optimum initial pH value was 9. 5 , and the optimum temperature was 20 °C ; for the removal of salt and alkali by the strain, the optimum temperature was 30-35 t.关键词
耐盐碱菌株/分离筛选/盐碱耐受力/生物学特性/盐碱去除Key words
saline-alkali tolerant bacterial strain/ isolation and screening/ saline-alkali tolerance/ biological characteristics/ salt-alkali removal.分类
农业科技引用本文复制引用
燕红,钟方,高新亮,刘蕊..耐盐碱菌株的分离筛选及生物学特性和盐碱去除效率的研究[J].生态学杂志,2012,31(4):1000-1008,9.
