陕西医学杂志2012，Vol.41Issue(5)：515-518,4.
低氧浓度改变对神经干细胞增殖分化及HIF-1α表达的影响
Effects of hypoxic concentration on the proliferation and differentiation of neural stem cells and the expression of the HIF-1α
摘要
Abstract
Objective:To observe the proliferation and differentiation of cortical neural stem cells (NSCs) from embryonic mice and the expression of hypoxia induciblc factor-la( HIF-la) under the different hypoxic concentrations in vitro, and to analyze the contribution of HIF-laon NSCs. Methods: NSCs from brain cortex of embryonic mice were cultured and ampiificatcd in lower O2 ( 3 % O2 and 10 % O2) in vitro , and all experimental dishes were divided into hypoxia for 1 , 3 and 5 days group ; The normoxic group was as control. Immunocytochcmical techniques were used to identify stem cells and their progenies , HIF-la mRNA expression in NSCs was examined by RT-PCR. Results : HIF-la mRNA was expressed significantly higher in every hypoxic group, and 10 % O2 group compared with 3 % O2 group, HIF-la mRNA expression increased significantly in hypoxia 5d . The ratio of NSE-positivc neurons in the experimental group were significantly higher than the control group , and 10 % O2 group compared with 3 % O2 group , the ratio of NSE-positivc neurons was significantly higher. Conclusion : Hypoxia can promote the proliferation and differentiation of NSCs and the expression of HIF-la mRNA , and the expression of HIF-la mRNA with hypoxia concentration and time-related.关键词
细胞分化/干细胞/代谢/基因/代谢/细胞低氧/模型,动物/大鼠/HIF-1αKey words
Cell differentiation/ Stem cell/metabolism/ Gene/metabolism/ Cell hypoxia/ Models/ animal/ Rats/ @ HIF-la分类
医药卫生引用本文复制引用
冯娜,彭兴春,贺细菊,戴冀斌,张涛..低氧浓度改变对神经干细胞增殖分化及HIF-1α表达的影响[J].陕西医学杂志,2012,41(5):515-518,4.基金项目
湖北医药学院硕士启动金项目(K1200801) （K1200801）
