实用医学杂志2012，Vol.28Issue(5)：708-711,4.DOI:10.3969/j.issn.1006-5725.2012.05.008
Prdx2在促性腺激素预处理的小鼠卵巢中的表达
Expression pattern of Prdx2 in the gonadotropin-primed immature mice ovary
摘要
Abstract
Objective To detect the ovarian expression of Prdx2 during follicle development, maturation and ovulation in the gonadotropin primed immature mice. Methods 75 immature C57/BL6 mice were randomly allocated into 14 groups (control group: 10 mice, treated groups: 5 mice/group). The expression of Prdx2 was detected by realtime PCR and Western blot at the time points of 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 24 h and 48 h after PMSG treatment, 5 h, 8 h, 10 h, 12 h and 24 h after injecting of Hcg for inducing ovulation. Results The real-time PCR data showed that the Mrna level in the treated groups were significantly reduced than those in the control group after the injection of PMSG. At 2 h, its expression appeared the lowest, then increased gradually and reached a peak at 24 h, but still lower than the control group. Significant differences were found between the control group (CO, the group treated with sodium chloride for 2 h) and the groups treated with PMSG for 2h, 4h, 6h, 8h, 10 h, 12 h, 48 h, respectively (P < 0.05), yet there was no significant difference between the C0 group and the 24 h group (P = 0.238). At the same time, we found that there was significant difference between the 2 h group and the 6 h group (P = 0.00), but the other groups had no significant difference compared to each other(P> 0.05). The mice ovulation were induced by injecting Hcg after dealing with PMSG, the expression of Prdx2 in the groups after injecting Hcg were significantly downregulated when compared to the control group (Cl, the group treated with sodium chloride for 48 h, P < 0.05), yet had no clear difference as compared with the group of P48 (the group with PMSG for 48 h, P > 0.05). Conclusion Prdx2 probably plays an important role in follicle development, maturation and ovulation.关键词
卵巢/Prdx2/孕马血清促性腺激素/人绒毛膜促性腺激素/卵泡发育/排卵/小鼠Key words
Ovary/Prdx2/PMSG/Hcg/Follicle development/Ovulation/Mice引用本文复制引用
杨书红,王硕,袁明,罗爱月,沈薇,丁婷,赖志文,王世宣..Prdx2在促性腺激素预处理的小鼠卵巢中的表达[J].实用医学杂志,2012,28(5):708-711,4.基金项目
卫生部公益性行业科研专项项目(编号:200802159) （编号:200802159）
教育部高等学校博士点科研基金项目(编号:200804870009) （编号:200804870009）
国家自然科学基金资助项目(编号:30973148) （编号:30973148）
