实用医学杂志2012，Vol.28Issue(5)：825-827,3.DOI:10.3969/j.issn.1006-5725.2012.05.055
荧光原位杂交在慢性粒细胞白血病细胞BCR/ABL融合基因检测中的应用及其意义
The detection of BCR/ABL fusion gene by interphase fluorescence in situ hybridization in patients with chronic myeloid leukemia and its clinical significance
摘要
Abstract
Objective To explore the clinical significance of the detection of BCR/ABL fusion gene in bone marrow and peripheral blood cells by fluorescence in situ hybridization (FISH) in patients with chronic myeloid leukemia (CML). Methods FISH technique was used to detecte BCR/ABL fusion gene in bone marrow and peripheral blood cells in healthy controls and CML patients. Results The positively expressing rates of BCR/ABL fusion gene in peripheral blood and bone marrow cells were (61.9 ± 22.3)% and (68.4 ± 19.8)% in patients with chronic phase of CML, were (77.2 ± 16.7)% and (86.8 ± 12.1)% in those with accelerating phase, and were (80.6 ± 17.5)% and (81.4 ± 18.0)% in those with blastic crisis, significant difference was not observed (P > 0.05). A significant correlation was found between the BCR/ABL positive cells in peripheral blood and in bone marrow samples. Moreover, the higher rate of complete molecular remission was achieved by treating with imatinib than with interferon and hydroxyurea during complete remission phase (P < 0.05). Conclusions Detecting BCR/ABL fusion gene in peripheral blood and bone marrow samples by FISH are beneficial to diagnosis, treatment and minimal residual disease monitor in CML.关键词
白血病,髓系,慢性,BCR-ABL/阳性/荧光原位杂交/BCR/ABL/融合基因Key words
Leukemia,myelogenous,chronic,BCR-ABL positive/FISH/BCR/ABL/Fusion gene引用本文复制引用
张济,李君君,颜家运,邹礼衡,刘静..荧光原位杂交在慢性粒细胞白血病细胞BCR/ABL融合基因检测中的应用及其意义[J].实用医学杂志,2012,28(5):825-827,3.基金项目
湖南省教育厅科研基金资助项目(编号:10C1161) （编号:10C1161）
