实用医学杂志2012，Vol.28Issue(6)：874-876,3.DOI:10.3969/j.issn.1006-5725.2012.06.005
产甲酸草酸杆菌基因frc和oxc在人骨髓间充质干细胞中的表达
Expressions of frc and oxc genes of oxalobacter formigenes in human bone marrow mesenchymal stem cells
摘要
Abstract
Objective To investigate the expressions of oxalate-degredation genes frc and oxc of oxalobacter formigenes (OXF) in human bone marrow mesenchymal stem cells (hMSCs). Methods Genome of OXF saved in our laboratory was as the mould, the frc and oxc genes were amplified by PCR, and were cloned into retrovirus vectors pLEGFP-Nl and pBaBE-puro. The expressions of frc and oxc genes in hMSCs were detected by Q-PCR and Western blot. Results The recombinant retrovirus vectors pLEGFP-Nl-myc-frc and pBaBE-puro-flag-oxc were successfully constructed. The frc and oxc genes could be stably expressed at mRNA and protein level in hMSCs. Conclusion Frc and oxc genes could be transfected into normal hMSCs by retrovirus vectors successfully.关键词
高草酸尿/产甲酸草酸杆菌/骨髓间充质干细胞/逆转录病毒载体Key words
Hyperoxal urine/ Oxalobacter formigenes/ hMSCs/ Retrovirus vector引用本文复制引用
阳旭明,袁坚,雷鸣,张泽..产甲酸草酸杆菌基因frc和oxc在人骨髓间充质干细胞中的表达[J].实用医学杂志,2012,28(6):874-876,3.基金项目
广东省科技厅资助项目(编号:2009B080701026) （编号:2009B080701026）
国家自然基金资助项目(编号:30801140) （编号:30801140）
