实用医学杂志2012，Vol.28Issue(7)：1046-1048,3.DOI:10.3969/j.issn.1006-5725.2012.07.005
SV40LT抗原诱导人源性淋巴细胞恶性转化
SV40 large T antigen induces human-derived lymphocyte malignant transformation
摘要
Abstract
Objective To study the effects of SV40 large T on human-derived lymphocyte transformation. Methods Peripheral blood mononucleate lymphocytes were conducted with the retroviral vector plasmid pBabe-SV40 large T. The cells morphological changes were watched by inverted microscope. The protein of large T antigen was detected by Western blot. Lymphocyte surface molecules were identified by flow cytometry. The cell karyotype was analyzed by G-banding. The cell proliferation was described by growth curve. The soft agar assay was performed to examine the cells malignant transformation. Results Some significant morphological changes were observed in the SV40LT gene transfected human-derived lymphocytes is accompanied by transition from suspension to adherent growth. The expression of large T antigen was found in the transfected cells. Moreover, 67% cells showed CD56 marker and were able to grow in soft agar. Conclusion SV40 large T antigen successfully induced a stable passaging and malignant transformation of lymphocyte with expression of CD56+.关键词
SV40LT/人源淋巴细胞/永生化Key words
SV40LT/ Human-derived lymphocyte / Immortalized引用本文复制引用
彭艳兰,李道传,邢秀梅,魏青,张莹莹,王庆,陈雯,肖勇梅..SV40LT抗原诱导人源性淋巴细胞恶性转化[J].实用医学杂志,2012,28(7):1046-1048,3.基金项目
国家自然科学基金资助项目(编号:81072284) （编号:81072284）
卫生公益性行业科研专项(编号:200902006) （编号:200902006）
中山大学重大项目培育计划(编号:10ykjc05) （编号:10ykjc05）
