实用医学杂志2012，Vol.28Issue(7)：1184-1186,3.DOI:10.3969/j.issn.1006-5725.2012.07.061
多参数流式细胞术在儿童急性B淋巴细胞白血病微量残留病检测中的应用
Detection of minimal residual disease in children with B lineage acute lymphoblastic leukemia by multiparameter flow cytometry
摘要
Abstract
Objective To evaluate the application value of multiparameter flow cytometry (FCM) in screening minimal residual disease (MRD) in children with B lineage acute lymphoblastic leukemia (B-ALL). Methods On the basis of the leukemia-associated immunophenotypes (LAIPs) of 53 children with B-ALL, the MRD of 68 consecutive bone marrow specimens from 53 patients who achieved complete remission were detected by 4-color antibody combination, mainly CD19-APC/CD45-PerCP/CD10-FITC/CD34-PE ,CD45/SSC and CD45/CD19 gating FCM. Results Eighteen LAIPs were detected in 53 cases with B-ALL. Abnormal expression of CD45 , CD10, CD19, and CD34 were detected in 98.11% of the 53 patients. 12 patients were MRD positive and had 0.012%~0.55% of residual leukemia cells. 7 patients (58.3%) had the modulation of LAIPs, the LAIPs of 10 cases were monitored by the antigen panel of CD19/CD45/CD10/CD34. Conclusion According to the LAIPs of children with B-ALL, multiparameter FCM using 4-color antibody panel, including mainly CD19/CD45/CD10/CD34, is appropriate to detect MRD.关键词
伯基特淋巴瘤/急性B淋巴细胞白血病/微量残留病/流式细胞术/儿童Key words
Burkitt lymphoma/ B lineage acute lymphoblastic leukemia / Minimal residual disease / Flow cytometry/ Childhood引用本文复制引用
吴韶清,廖灿,谢闺娥,孙新,黄以宁,叶铁真,赖冬波..多参数流式细胞术在儿童急性B淋巴细胞白血病微量残留病检测中的应用[J].实用医学杂志,2012,28(7):1184-1186,3.基金项目
国家自然科学基金资助项目(编号:81102248) （编号:81102248）
广州市医药卫生科技项目(编号:201102A213045) （编号:201102A213045）
广州市妇女儿童医疗中心博士启动基金(编号:201012) （编号:201012）
