深圳大学学报（理工版）2012，Vol.29Issue(1)：73-79,7.DOI:10.3724/SP.J.1249.2012.01073
非生物胁迫因子对大豆Sali3-2基因的调控作用
Regulatable gene expression controlled by the promoter of Sali3-2 under different abiotic stresses
摘要
Abstract
Salii-2 from soybean is a BURP gene related to stress response. To elucidate its transcriptional regulation characteristics, the upstream non-coding sequence ( -1 945 / + 1) of Salii-2 was scanned in PLACE database for searching the cis-acting regulatory elements. Several copper response elements and some cis-element motifs related to gene induction by abscisic acid (ABA) , dehydration and cold stress were found in this sequence. The expression of reporter gene Gus driven by the promoter region of Salih-2 was further studied in tobacco cells and Arabidopsis seedlings at different abiotic stresses. Results show that the expression of the reporter is obviously up-regulated by Al3 + stress and intensely suppressed by abundance Cu2 +. The expression of the reporter is slightly up-regulated by abscisic acid, osmotic stress and salt stress in transgenic tobacco cells. These findings extend our understanding of the regulation of Salii-2 expression.关键词
生物信息学/基因调控/启动子/Sali3-2基因/铜响应元件/非生物胁迫/GUS染色Key words
bioinformatics/ gene regulation/ promoter/ Sali3-2 / copper response elements/ abiotic stress/ GUS analysis分类
生物科学引用本文复制引用
唐玉林,曹雁,欧忠华,杨悉妮,胡恋苹,郑易之..非生物胁迫因子对大豆Sali3-2基因的调控作用[J].深圳大学学报（理工版）,2012,29(1):73-79,7.基金项目
国家自然科学基金资助项目(30770184) （30770184）
