生殖医学杂志2012，Vol.21Issue(1)：8-11,4.DOI:10.3969/j.issn.1004-3845.2012.01.003
去唾液酸糖蛋白受体在乙型肝炎病毒感染者精子中表达的初步研究
Preliminary study on the asialoglycoprotein receptor involving in the procedure of HBV invading human sperm
徐婕 1方军 2靳镭 1彭芳昕 1刘世伟1
作者信息
- 1. 华中科技大学同济医学院附属同济医院,生殖医学中心,湖北武汉430030
- 2. 华中科技大学同济医学院附属同济医院输血科,湖北武汉430030
- 折叠
摘要
Abstract
Objective: To explore the mechanism of hepatitis B virus (HBV) invading into human sperm through detecting and comparing the expression of the asialoglycoprotein receptor( ASGP-R) in spermatozoa of HBV Infected male patients and the HBV-negative men.Methods: The expression levels of ASGP-R in HBV negative group(? = 35),single-positive (HBsAg + /HBeAg-) group (w = 28)and double-positive (HBsAg+/HBeAg+) group (m= 16)were detected with ASGP-R monoclonal antibodies by Western blot and flow cytometry.Results: ASGP-R existed in the plasma membrane and the cytoplasm of human sperm. ASGP-R was highly expressed in human sperm membrane and lowly expressed in human sperm cytoplasm. The expression levels of ASGP-R in negative group, single-positive group and double-positive group were increased gradually. There were significantly higher ASGP-R positive rates in single-positive group and double-positive group than that in negative group (P<0. 05).Conclusions: There was ASGP-R expression in the plasma membrane and the cytoplasm of human sperm. HBV might invade into the human sperm by ASGP-R which exist in the plasma membrane. HBV might increase the expression levels of ASGP-R by means of some mechanism.关键词
乙型肝炎病毒/精子/去唾液酸糖蛋白受体/流式细胞术/免疫印迹试验Key words
Hepatitis B virus/Sperm/Asialoglycoprotein receptor, Flow cytometry/Western blot引用本文复制引用
徐婕,方军,靳镭,彭芳昕,刘世伟..去唾液酸糖蛋白受体在乙型肝炎病毒感染者精子中表达的初步研究[J].生殖医学杂志,2012,21(1):8-11,4.
