生殖医学杂志2012，Vol.21Issue(1)：12-16,5.DOI:10.3969/j.issn.1004-3845.2012.01.004
淋巴细胞主动免疫治疗对原因不明性复发性流产患者Treg及Th17细胞因子的影响
Study on the effective of lymphocyte immunotherapy on Treg and Thl7 cytokines in unexplained recurrent spontaneous abortion patients
刘长明 1丛林 1袁静 1方慧琴 1陈薇1
作者信息
- 1. 安徽医科大学第一附属医院妇产科产前诊断中心,合肥230022
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摘要
Abstract
Objective: To investigate the effect of lymphocyte immunotherapy on transcriptional (Foxp3, ROR-yt) and functional (EBi3, IL-17A) cytokines of Treg and Thl7 cells in unexplained recurrent spontaneous abortion patients.Methods: Twenty patients suffered with unexplained recurrent spontaneous abortion were treated with active lymphocyte immunotherapy for 3 times. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect Foxp3 and ROR-yt mRNA in peripheral blood. Enzyme-linked immunosorbent assay (ELISA) was used to measure IL-17 and IL-35 levels in serum.Results: The relative expressions of Foxp3 mRNA were 0. 28±0. 06 and 0. 52 + 0. 04 before and after the treatment, and 0. 60±0.11 and 0. 42±0. 09 for ROR-yt mRNA. While the serum levels of IL-17 were (16.12±4.16) ng/L and (14. 72±1. 98) ng/L, IL-35 levels as (11. 53±2. 26) ng/L and (26. 34±2. 77) ng/L. All the differences before and after treatment were statistically significant (F<0. 05).Conclusion: The levels of cytokines of Treg and Thl7 cells were significantly different after treatment. The levels of Treg cytokines were changed more pronounced. It suggested that Treg cells might play more important role in the outcome of pregnancy.关键词
复发性流产/淋巴细胞主动免疫治疗/细胞因子Key words
Recurrent spontaneous abortion/Lymphocyte immunotherapy/Cytokines引用本文复制引用
刘长明,丛林,袁静,方慧琴,陈薇..淋巴细胞主动免疫治疗对原因不明性复发性流产患者Treg及Th17细胞因子的影响[J].生殖医学杂志,2012,21(1):12-16,5.
