特产研究2012，Vol.34Issue(1)：14-18,5.
坏死梭杆菌斑马鱼模型建立与LD50的测定
Establishment of Zebrafish Model with Fusobacterium Necrophorum Biotype AB and Determination of LD50
摘要
Abstract
To establish zebrafish model infected with Fusobacterium Necrophorum Biotype FN (AB) artificially, zebrafish were divided, and were given intraperitoneal injection with FN (AB) isolate from cattle in different dosages of 3 × 1010cfu/mL, 3 × 109cfu/mL, 3 × 108cfu/mL, 3 × 107cfu/mL, respectively. The morbidity and mortality of zebrafish were monitored in fixed time to establish the infectious animal modeL The 50% lethal dose of FN(AB) in zebrafish (LD50) was 1.06 × 106 cfu / mL Side swimming, as-cites, red and swollen in the injection site was observed in the infected Zebrafish. There were also obvious pathological changes in liver, renal, and intestinal tract of the infected zebrafish. Therefore, zebrafish can be an animal model infected with FN(AB).关键词
坏死梭杆菌/斑马鱼/动物模型/半数致死量Key words
Fusobacterium necrophorum/Zebrafish/Animal model/50% lethal dose分类
生物科学引用本文复制引用
曹阅,陈立志,刘晓颖,冯二凯,汪孙杰,徐晶,冯卓..坏死梭杆菌斑马鱼模型建立与LD50的测定[J].特产研究,2012,34(1):14-18,5.基金项目
吉林省科技发展计划项目(20110223) （20110223）
吉林省科技发展计划项目(201205028) （201205028）
