土壤学报2012，Vol.49Issue(1)：36-42,7.
Btk杀虫蛋白在红壤和棕壤中的吸附热力学研究
ADSORPTION THERMODYNAMICS OF BTK INSECTICIDAL PROTEIN IN RED SOIL AND BROWN SOIL
摘要
Abstract
Adsorption isotherms of the insecticidal protein of Bacillus thuringiensis subsp. Kurstaki ( Btk) in red soil and brown soil were determined using the equilibrium adsorption method. Based on the isothems, their adsorption equilibrium constants and thermodynamic parameters were calculated. Results show that in the range between 278K and 318K, the Btk protein adsorption isotherms in red soil and brown soil followed the Langmuir equation ( R2 > 0.994 1) , showing that Bt adsorption and adsorption equilibrium constant decreased with rising temperature, but separation factor constant (RL) increased in the range of 0.218 1 ~0.580 1 in red soil and of 0.361 7 ~0.754 1 in brown soil, indicating that both were of preferential adsorption. Btk protein adsorption in the two soils was a spontaneous, exothermic, entropy-increasing process. In red soil the activation energy of adsorbed Btk protein ranged from 13.43 ~ 14.78 kJ mol-1 , while in brown soil it did from 10. 89 - 11.47 kJ mol-1 . Under rising temperature, it increased along with adsorption free energy, which suggests that the adsorption of Btk protein by the soils is turning from a physical process into a chemical one.关键词
苏云金芽胞杆菌/杀虫蛋白/红壤/棕壤/吸附/热力学Key words
Bacillus thuringiensis/ Insecticidal protein/ Red soil/ Brown soil/ Adsorption/ Thermodynamics分类
农业科技引用本文复制引用
周学永,刘祥云,董庆洁,黄巧云,刘惠芬,丛方地,崔新仪..Btk杀虫蛋白在红壤和棕壤中的吸附热力学研究[J].土壤学报,2012,49(1):36-42,7.基金项目
国家自然科学基金项目(31070478)、天津市自然科学基金重点项目(08JCZDJC18800)和天津农学院科研计划项目(2008D016)联合资助 （31070478）
