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L-亮氨酸-U-13C6的研制

张亮 杜晓宁 李良君

同位素2011,Vol.24Issue(z1):106-111,6.
同位素2011,Vol.24Issue(z1):106-111,6.

L-亮氨酸-U-13C6的研制

Preparation of L-leucine-U-13C6

张亮 1杜晓宁 1李良君1

作者信息

  • 1. 上海化工研究院上海稳定同位素工程技术研究中心,上海200062
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摘要

Abstract

With yellow Arthrobacter as starting strain and through mutation breeding, mutant strain TS151 is available to us . The dissertation focuses on the mutation breeding prescription , fermentation condition and purification of L-leucine high-yielding strain TS151. The results show: With 92 h cultivation in 220 r/min rotation speed and 28 ℃ ,the acid production of high abundance is more than 15 g/L, extraction yield is about 70%. As products, the isotopic abundance of stable isotope labeled L-leucine is 97. 03%, the purity is more than 98%.

关键词

13C/标记/L-亮氨酸

Key words

13C/label/L-leucine

分类

化学化工

引用本文复制引用

张亮,杜晓宁,李良君..L-亮氨酸-U-13C6的研制[J].同位素,2011,24(z1):106-111,6.

同位素

OACSTPCD

1000-7512

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