同位素2011,Vol.24Issue(z1):106-111,6.
L-亮氨酸-U-13C6的研制
Preparation of L-leucine-U-13C6
张亮 1杜晓宁 1李良君1
作者信息
- 1. 上海化工研究院上海稳定同位素工程技术研究中心,上海200062
- 折叠
摘要
Abstract
With yellow Arthrobacter as starting strain and through mutation breeding, mutant strain TS151 is available to us . The dissertation focuses on the mutation breeding prescription , fermentation condition and purification of L-leucine high-yielding strain TS151. The results show: With 92 h cultivation in 220 r/min rotation speed and 28 ℃ ,the acid production of high abundance is more than 15 g/L, extraction yield is about 70%. As products, the isotopic abundance of stable isotope labeled L-leucine is 97. 03%, the purity is more than 98%.关键词
13C/标记/L-亮氨酸Key words
13C/label/L-leucine分类
化学化工引用本文复制引用
张亮,杜晓宁,李良君..L-亮氨酸-U-13C6的研制[J].同位素,2011,24(z1):106-111,6.