物理化学学报2012，Vol.28Issue(2)：470-478,9.DOI:10.3866/PKU.WHXB201112051
超声作用下稀土离子对纳米TiO2与牛血清白蛋白相互作用的影响
Effects of Rare Earth Ions on the Interaction between Nano TiO2 and Bovine Serum Albumin in the Presence of Ultrasound
摘要
Abstract
The effects of rare earth ions (La3*, Gd3*, Yb3*) on the interactions between nano TiO2 and bovine serum albumin (BSA) were investigated in the presence of ultrasound. A combination of ultraviolet (UV) spectroscopy, fluorescence spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize the interactions under simulated human physiological conditions. The endogenous fluorescence of BSA was quenched by nano TiO2 in different systems. The mechanism of fluorescence quenching was static quenching with non-radiative energy transfer. SDS-PAGE revealed that the structure of BSA was not obviously destroyed upon binding with nano TiO2 in different systems. Hydrogen bond and van der Waals interaction were deduced, on the basis of the thermodynamic parameters, to be the major driving forces. The binding distance of nano TiO2 to BSA and the thermodynamic parameters were changed in the presence of rare earth ions. However, the number of binding sites and the type of intermolecular force remained essentially unchanged. This indicated that the interaction between nano TiO2 and BSA was influenced by the rare earth ions, and that a lanthanide tetrad effect was observed. It is conjectured that rare earth ions participate in the nano TiO2-BSA interaction process by means of "ionic bridge" formation or "appositional substitution".关键词
超声/纳米TiO2/牛血清白蛋白/稀土离子/结合作用Key words
Ultrasound/Nano TiO2/Bovine serum albumin/Rare earth ion/Interaction分类
化学化工引用本文复制引用
高晓亚,文雯,宋志英,张爱平,郝娟,黄茜..超声作用下稀土离子对纳米TiO2与牛血清白蛋白相互作用的影响[J].物理化学学报,2012,28(2):470-478,9.基金项目
山西省自然科学基金(2010011048-1)和山西医科大学科技创新基金(01200806)资助项目 （2010011048-1）
