西安交通大学学报（医学版）2012，Vol.33Issue(2)：199-202,4.
IL-17A在博来霉素致小鼠肺纤维化形成中的表达
Expression of IL-17A in bleomycin-induced pulmonary fibrosis model
摘要
Abstract
Objective To explore the expression of interleukin-17A (IL-17A) during pulmonary fibrosis. Methods Forty male C57BL/6 mice were randomized as normal control group (N group) and bleomycin-induced pulmonary fibrosis model group (BLM group) with 20 in each. Mice were administered with bleomycin intratracheally at a dose of 5 mg/kg in BLM group and intratracheally administered with 0.9% saline of the same dose in N group. Then they were sacrificed on days 3, 7, 14 and 28. IL-17A in lung tissues was measured by semi-quantitative RT PCR and immunohistochemisty. The number of CD4+IL-17+T cells in the spleen was measured by flow cytometry. Results ①IL-17A was located in the terminal bronchioles, respiratory bronchioles and alveolar epithelia. ②IL-17A began to increase on day 7, reached the peak on day 14 and then began to decrease on day 28, which was all higher than in normal group. ③The pattern of changed number of CD4+ IL-17+ T/CD4+ T cells from spleen lymphocytes was the same as the amount of IL-17A expression. Conclusion The expression of IL-17A is up-regulated in bleomycin-induced pulmonary fibrosis in mice. IL-17A may be involved in the development and progression of pulmonary fibrosis.关键词
肺纤维化/Th17/白细胞介素17/博来霉素Key words
pulmonary fibrosis/ Thl7/ interleukin 17/ bleomycin分类
医药卫生引用本文复制引用
董昭兴,邰文琳,雷雯,邹华,付立主,张涛,王殿华..IL-17A在博来霉素致小鼠肺纤维化形成中的表达[J].西安交通大学学报（医学版）,2012,33(2):199-202,4.基金项目
云南省科技厅应用基础面上项目(No.2009ZC107M),云南省教育厅科学研究基金重点项目(No.2010Z029) （No.2009ZC107M）
