西北林学院学报2012，Vol.27Issue(2)：98-101,4.DOI:10.3969/j.issn.1001-7461.2012.02.21
花椒愈伤组织EMS诱变及变异研究
Variation and EMS Mutagenesis in Callus of Zanthoxylum bungeanum
摘要
Abstract
The callus of phloem and leaves of Zanthoxylum bungeanum were treated by ethyl methane sul-fonate (EMS) to determine the semi-lethal concentration of EMS and to get suitable source of callus. The results showed that the semi-lethal concentrations of EMS were 0. 3% (with 6 h treatment) , 0. 5% (4h) , 0. 7% (2h). From the regeneration plants which had been mutated, we found numerous variations such as the length and width of the leaves. Through examining the morphological characteristics, 340 mutation plants were selected.关键词
花椒/愈伤组织/EMS/变异Key words
Zanthoxylum bungeanum/ callus/ ethyl methane sulfonate (EMS)/ variation分类
农业科技引用本文复制引用
薛惠丹,李周岐..花椒愈伤组织EMS诱变及变异研究[J].西北林学院学报,2012,27(2):98-101,4.基金项目
陕西省“13115”科技创新工程项目(2007ZDKG-12) （2007ZDKG-12）
西北农林科技大学唐仲英育种基金项目. （）
