陕西科技大学学报(自然科学版)2012,Vol.30Issue(1):4-7,4.
薄层层析-紫外分光光度法测定银杏茶中银杏酸含量
DETERMINATION OF GINKGOLIC ACIDS IN GINKGO TEA BY TCL-UV SPECTROPHOTOMETRY
摘要
Abstract
To establish a fast determination method of ginkgolic acids(Gas) in ginkgo tea by TLC-UV. The sample through hexane Soxhlet extraction and TCL purification which was accomplished on G plate by using the mobile phase of petroleum ether : ethyl acetate : glacial acetic acid (18 : 1 : l),then determined by UV spectrophotometry at 307 nm. The results showed that there was a linear relationship within the range of 0. 009 - 0. 093 mg/mL(R2 = 0. 999 7) , the RSD was 2. 15% ,the average recovery was 100. 90% ,the results being detected by UV spectrophotometry were close to those by HPLC. The method is suitable for the Gas analysis in ginkgo tea.关键词
银杏茶/银杏酸/薄层层析/紫外分光光度法Key words
ginkgo tea/ ginkgolic acids/ TCL/ UV分类
轻工纺织引用本文复制引用
秦俊哲,郭云霞..薄层层析-紫外分光光度法测定银杏茶中银杏酸含量[J].陕西科技大学学报(自然科学版),2012,30(1):4-7,4.基金项目
陕西省重大科技创新专项项目(2010ZKC11-05) (2010ZKC11-05)