现代妇产科进展2012，Vol.21Issue(2)：101-104,4.
L型氨基酸转运蛋白抑制剂BCH联合顺铂对卵巢癌SKOV3细胞增殖的抑制作用
System L amino acid transporter inhibitor( BCH )in combination with cisplatin inhibits the proliferation of ovarian cancer SKOV3 cells
摘要
Abstract
Objective; To explore the effects of System L amino acid transporter inhibitor 2-aminobicyclo-(2,2,1 )-heptane-2-carboxylic acid ( BCH) alone or in combination with cisplatin on the proliferation of human ovarian cancer SKOV3 cells and the possible molecular mechanism. Methods; human SKOV3 cells were treated with different concentrations of BCH a-lone or combination with cisplatin, the cell viability was assessed by CCK-8 assay; Western blot analysis was performed to detect the affection of mTOR signal pathway by BCH. Results; BCH alone could significantly inhibit the proliferation of SK.OV3 cells in time- and dose- manners. Comparing with administration alone, the cell viability was decreased significantly when BCH was combined with cisplatin (P<0. 01 ) ,and the effect was more significant when BCH treatment followed cisplatin administration(P<0.05). Western blot analysis showed that BCH could inhibit mTOR signal pathway by decreasing the phosphorylation of mTOR,p70 S6K and 4E-BP1. Conclusion: Both BCH alone and in combination with cisplatin can significantly inhibit the proliferation of human ovarian cancer SKOV3 cells,it shows synergistic effects when BCH treatment followed cisplatin administration.关键词
L型氨基酸转运蛋白抑制剂/顺铂/SKOV3细胞/mTOR信号通路Key words
System L amino acid transporter inhibitor/Cisplatin/SKOV3 cells/mTOR signal pathway分类
医药卫生引用本文复制引用
赵姗,杨兴升,包香香,张心雨,孙晓燕,刘跃洋..L型氨基酸转运蛋白抑制剂BCH联合顺铂对卵巢癌SKOV3细胞增殖的抑制作用[J].现代妇产科进展,2012,21(2):101-104,4.基金项目
山东省科技发展计划基金项目(No:2011GSF12121) （No:2011GSF12121）
