现代妇产科进展2012，Vol.21Issue(2)：105-108,4.
阻断丝裂原活化蛋白激酶信号通路对子宫内膜癌生长抑制作用的研究
Inhibition effect of MAPK/ERK signaling pathway on endometrial cancer in vivo
摘要
Abstract
Objective: To explore whether blockade of the extracellular-regulated ki-nase (ERK) signaling pathway can inhibit the growth of xenografts of endometrial cancer cell lines with different estrogen receptors (ER) profiles in vivo. Methods: Human endometrial cancer Ishikawa bearing ER and HEC-1A with low ER status cells were subcutaneously injected into BALB/c nude mice to establish endometrial cancer xenograft tumor models. The effects of MAPK/ERK1/2 inhibitor PD98059 on the growth of the xenograft tumors and apoptotic state of 'Ishikawa and HEC-1A cells were tested in vivo using the inhibitory rate,the terminal deoxynu-cleotidyl transferase-mediated nick-end labeling assay, H/E-stain. Western blot analysis was used to detect the alterations of activated ERK ( P-ERK) during this process. Results: ( 1 ) PD98059 can significantly supress the growth of endometrial cancer xenografs. (2) HE staining showed that the proportion of nucleus/cytoplasm is smaller and have lower density of vesseles. TUNEL assay showed a higher apoptosis rate in the experimental groups. ( 3) Western blot showed that PD98059 can effectively reduce the expression of P-ERK in tumors. Conclusions; PD98059 shows remarkable inhibitory effects on xenograft tumors of endometrial carcinoma cell lines with different expression status of ER in vivo through blockage of the MAPK/ERK signaling pathway. This suggests that targeting this pathway may be an effective therapeutic strategy against endometrial neoplasms.关键词
裸鼠/子宫内膜肿瘤/PD98059/p-ERKKey words
Nude mice/Endometrial neoplasms/PD98059/Phosphorylated extracellu-分类
医药卫生引用本文复制引用
郭瑞霞,王新燕,张瑞芳,葛新,史惠蓉,乔玉环..阻断丝裂原活化蛋白激酶信号通路对子宫内膜癌生长抑制作用的研究[J].现代妇产科进展,2012,21(2):105-108,4.基金项目
河南省高校科技创新人才基金资助项目(No:2010HASTTTDD5) （No:2010HASTTTDD5）
