中国组织工程研究2012，Vol.16Issue(11)：1901-1905,5.DOI:10.3969/j.issn.1673-8225.2012.11.001
下颌骨牵引成骨过程中基因干预对牵引区转化生长因子β1表达的影响
Effects of gene therapy on the expression of transforming growth factor beta 1 during mandible distraction osteogenesis
摘要
Abstract
BACKGROUND: Although recent studies have shown that the local gene therapy can promote the formation of new bone indistraction gap, the effect of gene therapy on the expression of local growth factors is unclear.OBJECTIVE: To investigate the effect of electroporati on-mediated gene therapy on the expression of transforming growthfactor-β1 (TGF-β1) during mandibular distraction osteogenesis in a rabbit model.METHODS: Mandibular distraction of 0.8 mm/d was performed in New-Zealand rabbitsat 3 daysafter bilateral mandibularosteotomy and lasted for 7 days. After the completion of distraction, the rabbits were randomly divided into five groups. 2 μg(0.1 μg/μL)recombinantplasmidpIRES-hVEGF165-hBMP2, pIRES-hBMP2, pIRES-hVEGF165, pIRESand normal saline wasinjected into the distraction area respectively. After injection, everygroup employed electroporation.RESULTS AND CONCLUSION: Immunohistochemical staining showed thatTGF-β1 was mainly expressed in the cytoplasm.Seven days after transfection, the staining ofTGF-β1 was positive on osteocytes of bone edge, osteocytes of woven bone callusand osteoblasts on the surfaceof bone callus. At 14 days, the expression ofTGF-β1 wasfound on osteocytes of newborn wovenbone callus, osteoblasts on the surfaceof bone callus, stromal cellsin granulation tissue, mononuclear giant cellsandmult nucleated giant cell a At 28 days, the positive expression of TGF-β1 was decreased obviously. Compared with the injectionof pIRESand normal saline, the expression ofTGF-β1 was significantly higher after the injecti on of recombinantplasmidpIRES-hVEGF165-hBMP2,pIRES-hBMP2 and pIRES-hVEGF165 (P＜0.05 or P＜0.01). It suggests that gene therapy canpromote TGF-β1 expression effectively and promote the formation of new bone and cell matrix in distraction gap.关键词
电穿孔/基因疗法/骨生成/牵张/转化生长因子β1分类
医药卫生引用本文复制引用
何小川,李绍兰,胡纯兵,刘震,高志丹,尹康,吴国平,郭力..下颌骨牵引成骨过程中基因干预对牵引区转化生长因子β1表达的影响[J].中国组织工程研究,2012,16(11):1901-1905,5.基金项目
国家自然科学基金资助项目(30600653)；四川省应用基础研究计划项目(2006J13-128)；四川省卫生厅科研课题(060044，100285)。 （30600653）
