中国组织工程研究2012，Vol.16Issue(11)：1937-1940,4.DOI:10.3969/j.issn.1673-8225.2012.11.009
关节滑液对兔前交叉韧带重建后腱骨愈合生物力学和组织学的影响
Effect of joint synovial fluid on biodynamics and histology of tendon-bone healing after reconstruction of anterior cruciate ligament in rabbits
摘要
Abstract
BACKGROUND: At present, effect of joint synovial fluid on the strength of graft and tendon-bone healing after reconstruction ofanterior cruciate ligament (ACL) is inconclusive.OBJECTIVE: To observe the effect of joint synovial fluid on biodynamicsand histology of tendon-bone healing afterreconstruction of ACL in the rabbits.METHODS: Semitendinosus tendons were taken from lower extremities of New Zealand white rabbits to reconstruct ACLsimulation synovial fluid influence model with ipsilateral semitendinosus tendons, and contralateral semitendinosus tendons wereused for implanting "U"-shaped on the femoral condyle to avoid the influence of the synovial fluid. Specimens were collected at 4weeks after operation forbiomechanical measurement and histological observation.RESULTS AND CONCLUSION: Biomechanical measurement at 4 weeks after reconstruction showed that the average load of"U" shaped implanted tendon breakage was significantly higherthan that of the ACL reconstructed tendon (P<0.01). Histologicalobservation showed that necrotic tendon tissues in bone tunnel were replaced by new bone tissues and fibrous tissues.Sharpey's fibers and fibrocartilages were appeared on the tendon-bone interface. The number of bone cells on the tendon boneinterface of the "U" shaped implanted tendon was significantly higher than that of ACL reconstructed tendon (P <0.01),superior to intra-articular group. It results showed that biomechanics and tendon bone healing of the "U" shaped implantedtendon were both superior to the ACL reconstructed tendon. It is indicated that joint synovial fluid of ACL reconstruction has anadverse effecton ligament strength and tendon bone healing.关键词
关节滑液/前交叉韧带重建/半腱肌腱/腱骨愈合/生物力学/组织学分类
医药卫生引用本文复制引用
董向辉,凌鸣,冯伟楼,田昕..关节滑液对兔前交叉韧带重建后腱骨愈合生物力学和组织学的影响[J].中国组织工程研究,2012,16(11):1937-1940,4.基金项目
2008年陕西省"13115"科技创新工程重大科技专项(2008ZDKG-57). （2008ZDKG-57）
