中国组织工程研究2012，Vol.16Issue(11)：1963-1968,6.DOI:10.3969/j.issn.1673-8225.2012.11.015
一种适合肝细胞生长的无血清培养基
A new serum-free medium for hepatocytes growth
摘要
Abstract
BACKGROUND: A medium both for cell growth and avoiding adding serum medium is imminent, so serum-free medium emergeas the times require.OBJECTIVE: To develop a new serum-free medium for C3A cells to lay the foundation for the clinical application of hepatocytesand bioartificial liver.METHODS: C3A cells were cultivated with serum-free medium, complete medium and basal medium, respectively. The growthand morphological characteristics of cells were observed. Curves of cell growth and the energy were drawn. The leakage volumeof alaninetransaminase, urea level and the level of albumin were determined by automatic biochemistry analyzer andradioimmunoassay. The changes of gene expression were determined by real-time fluorescence quantitative PCR.RESULTS AND CONCLUSION: The cells in the three kinds of mediums grew well. Apart from the 7th day, the cell density had nosignificant difference between the serum-free medium and complete medium groups in the culture period (P＞0.05), whereas itwas obviously higher in these two groups than in the basal medium group at 4-7 days (P＞0.05). The level of urea in theserum-free medium group was significantly higher than that in the other two groups, and the level of urea in the complete groupwas higher than that in the basal medium group (P＜0.05). Real-time fluorescence quantitative RCR revealed that geneexpressions of CK18, CK19 and hepatocyte nuclear factor 4α were lower in the serum-free group as compared with the completemedium group (P＜0.05). The results showed that the new serum-free medium for hepatocytes is effective just like theconventional complete medium.关键词
培养基/无血清培养/C3A细胞/肝细胞/生物人工肝/PCR检测分类
医药卫生引用本文复制引用
赵逸超,汪艳,卢海,简国登,潘明新,高毅..一种适合肝细胞生长的无血清培养基[J].中国组织工程研究,2012,16(11):1963-1968,6.基金项目
国家高技术研究发展计划(863计划):No.2006AA02A141,人源细胞混合型生物人工肝的研制与开发. （863计划）
