中国组织工程研究2012，Vol.16Issue(11)：1969-1972,4.DOI:10.3969/j.issn.1673-8225.2012.11.016
成年大鼠心肌细胞的急性分离方法
Emergent isolation of myocardial cells from adult rats
摘要
Abstract
BACKGROUND: To obtain high survival rate and good activity of the normal single myocardial cells is the basis of myocardialelectrophysiology. It is important to find an experimental method which is close to the normal myocardial cells in morphology,function and structure.OBJECTIVE: To discuss the influence factors during emergent isolation of myocardial cells by using enzymatic method in ratsand to build an emergent isolation method for stable obtaining myocardial cells to provide cells for myocardial electrophysiologyand molecular biology.METHODS: The heart of Sprague-Dawley adult rats was rapidly excised after cervical dislocated and mounted on a Langendorffapparatus for aortic counter-current perfusion. It was perfused retrograde via the aorta for 5 minutes with calcium-free tyrode, andthen perfused with collagenase dissolved in nominally calcium-free tyrode for 20 minutes. Ultimately calcium solution wasperfused for 3 minutes. Then the rat ventricular cells were stored in HK solution.RESULTS AND CONCLUSION: Viability of freshly isolated myocardial cells which were rod-shaped with clear-striation was60%-80%. It is shown that to adjust and control all the factors in the emergent isolation can ensure the quantity and quality ofmyocardial cells.关键词
心肌细胞/成年大鼠/分离/数量/影响因素分类
医药卫生引用本文复制引用
韦丽兰,莫书荣..成年大鼠心肌细胞的急性分离方法[J].中国组织工程研究,2012,16(11):1969-1972,4.基金项目
广西科学基金项目(0728140),课题名称:红景天苷影响大鼠心肌细胞工作的机制的研究. （0728140）
