摘要
Abstract
BACKGROUND: Phyllanthus Emblica has a significant hypoglycemic effect. However, its mechanism is not clear.OBJECTIVE: To explore the effects of Phyllanthus Emblica extracts on the proteins related to the insulin signal pathway in ratbone and fat tissues.METHODS: A total of 30 SD rats were randomly divided into control group, model group and Phyllanthus Emblica group. Rats inthe latter two groups were intraperitoneally injected with streptozotocin to construct diabetic model. Rats in the PhyllanthusEmblica group were administrated with Phyllanthus Emblica extracts by gavage for 6 weeks; rats in the model group and thecontrol group were administrated with distilled water of the same volume by gavage.RESULTS AND CONCLUSION: Compared with the control group, the body mass, fasting blood glucose, fasting insulin andinsulin resistance index in diabetic rats increased significantly (P＜0.05); while the mRNA and protein levels ofphosphatidylinositol-3-kinase, protein kinase B and glucose transporter 4 in the fat and muscle tissues decreased significantly(P＜0.05, P＜0.01). The body mass, fasting blood glucose, fasting insulin and insulin resistance index in the Phyllanthus Emblicarats at 6 weeks after the gavage of Phyllanthus Emblica extracts was lower than those of the model rats; while the mRNA levels ofphosphatidylinositol-3-kinase, protein kinase B and glucose transporter 4 in fat and muscle tissues increased significantly(P＜0.01, P＜0.05). The protein expression of glucose transporter 4 in the fat tissues increased (P＜0.01). However, there wasno significant difference between the protein expressions of glucose transporter 4 in the muscles. These findings indicate thatPhyllanthus Emblica extracts can achieve hypoglycemic effects by regulating insulin-mediated phosphatidylinositol-3-kinase/protein kinase B/glucose transporter protein 4 signal transduction pathway.关键词
余甘子提取物/胰岛素抵抗/骨骼肌/蛋白激酶B/葡萄糖转运蛋白4分类
医药卫生
