中国组织工程研究2012，Vol.16Issue(11)：2015-2018,4.DOI:10.3969/j.issn.1673-8225.2012.11.027
QIAamp及Biomed粪便细菌基因组DNA提取试剂盒提取人肠道细菌基因组DNA质量的差异
Quality differences of bacterial genome DNA extracted from the human intestine by using QIAamp and Biomed DNA Stool Mini Kits
摘要
Abstract
BACKGROUND: Several studies have showed that there are different qualities of bacterial genome DNA extracted from the humanintestine using different kits. Therefore, it is essential and urgent to select an effective, simple, and excellent DNA stool mini kit.OBJECTIVE: To compare the quality differences of bacterial genome DNA extracted from the human intestinal by using QIAampand Biomed DNA Stool Mini Kits.METHODS: Thirty fresh fecal samples of healthy adult people were selected, and bacterial genome DNA was extracted usingthese two kits. Concentration, absorbance radio of 260 to 280 nm and extraction rate were measured. Species-specific primersfor Lactobacillus group were designed by a set of 16S rDNA-targeted to conduct the general PCR using the genome DNA as thetemplate. The electrophoresis strip's number, brightness and density were compared after gel electrophoresis; subsequently, thenumber of Lactobacillus group was detected by fluorescent real-time PCR.RESULTS AND CONCLUSION: Concentration, extraction rate, expression level, Lactobacillus amount of bacterial genomeDNA extracted from the human intestinal using QIAamp kit were higher than those using Biomed kit (P＜0.05 or P＜0.01). Itsuggests that the quality of genome DNA extracted with QIAamp kit is more excellent than that with Biomed kit.关键词
基因组DNA/QIAamp/试剂盒/Biomed/试剂盒/粪便细菌/乳酸菌属/实时荧光定量聚合酶链反应/组织工程分类
医药卫生引用本文复制引用
宋美茹,姚萍,张跃新..QIAamp及Biomed粪便细菌基因组DNA提取试剂盒提取人肠道细菌基因组DNA质量的差异[J].中国组织工程研究,2012,16(11):2015-2018,4.基金项目
新疆维吾尔自治区重点学科(内科学)建设基金(2001-2011). （内科学）
