中国组织工程研究2012，Vol.16Issue(11)：2028-2032,5.DOI:10.3969/j.issn.1673-8225.2012.11.030
微透析和高效液相色谱检测万古霉素在清醒兔眼玻璃体内的浓度
Microdialysis and high performance liquid chromatography detection for vancomycin concentration in vitreous chamber of conscious rabbits
王亚玲 1王洪格 1陈晓隆2
作者信息
- 1. 青岛市市立医院眼科,山东省,青岛市,266001
- 2. 中国医科大学附属盛京医院眼科,辽宁省,沈阳市,110006
- 折叠
摘要
Abstract
BACKGROUND: There are currently few studies regarding the pharmacokinetics of vancomycin via intravitreous injection.OBJECTIVE: To determine the concentration of vancomycin injected into the vitreous chamber of conscious rabbits.METHODS: A microdialysis probe was implanted into vitreous chamber of normal rabbit eyes and rabbit eyes infected withbacterial endophthalmitis for 24 hours, and 10 g/L vancomycin 0.1 mL was administered intravitreally. The drug concentration inthe vitreous chamber of rabbit eyes was determined at 0.5, 1, 2, 4, 6, 12, 24, 48, 72 and 84 hours after injection, through themicrodialysis and high performance liquid chromatogram-ultraviolet detection.RESULTS AND CONCLUSION: The metabolism of vancomycin showed an open two-compartment model in normal rabbit eyes.Its half-life was 51.66 hours and the peak concentration was 695.92 mg/L. The metabolism of vancomycin in the infected vitreouschamber showed a one-compartment model. Its half-life was 11.91 hours and the peak concentration was 713.35 mg/L. All rabbitswere injected with drugs for 84 hours and the intravitreous concentration of vancomycin was higher than minimal inhibitoryconcentration. The experimental findings indicate that microdialysis coupled to high performance liquid chromatography is apowerful tool to investigate the ocular pharmacokinetics of vancomycin, and the samples are harvested in a real-time, continuousand dynamic fashion when the experimental animals are conscious.关键词
微透析技术/高效液相色谱法/玻璃体/万古霉素/药代动力学分类
医药卫生引用本文复制引用
王亚玲,王洪格,陈晓隆..微透析和高效液相色谱检测万古霉素在清醒兔眼玻璃体内的浓度[J].中国组织工程研究,2012,16(11):2028-2032,5.
