中国组织工程研究2012，Vol.16Issue(14)：2471-2476,6.DOI:10.3969/j.issn.1673-8225.2012.14.001
四种诱导剂诱导骨髓间充质干细胞向心肌样细胞的分化
Induced differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by four inductors
刘博武 1吕安林 1黄炜 1侯婧 1侯兆蕾 1侯红 1达晶 1杨娜1
作者信息
- 1. 解放军第四军医大学西京医院心内科,陕西省西安市,710032
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摘要
Abstract
BACKGROUND: It has been rarely reported that different inductors are used to induce bone marrow mesenchymal stem cells todifferentiate into cardiomyocyte-hke cellsOBJECTIVE: To investigate the effects of 5-azacytidne, angiotensin Ⅱ, bone morphogenetic protein 2, pifithnn-o: on induceddifferentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.METHODS: Passage 3 rat bone marrow mesenchymal stem cells were divided into five groups control, 5-azacytidine,angiotensin Ⅱ,bonemorphogenetic protein 2.pifithrin-α.Surfacce antigen expression of bone marrow messnchymal stem cellswas identified by flow cytometry After 4 weeks of induction, Cx43 and troponin Ⅰ expression was detected by western blotmethod and calciumtransient capability of induccced bone marrow mesemmchymal stem cells wsa detected by laser confocal scanning microscopy with flou 3/AM.RESULTS AND CONCLUSION: Cell surface antigen CD29, CD44, CD45 positive rate was 89 1%, 90 4%, 1 9% respectively TroponinⅠexpression appeared in each group and the expression was significantly higher in the pifithrin-α group (P< 0. 05), as well as significantly lower in the bone morphogenetic protein 2 group (P < 0 05), compared with the remaining groups Cx 43 expression appeared in each group, and the expression was significantly lower in the control group (P < 0 05) compared with the remaining groups In the four experimental groups, Cx43 expression was highest in the pifithnn-α group and lowest in the angiotensin Ⅱ group (P < 0. 05) Calcium transient capability determined by fluorescence intensity was arranged as follows angiotensin Ⅱ group > pifithrin-α group > 5-azacytidine group > bone morphogenetic protein 2 group > control group (P < 0 01) These findings suggest that after induced by 5-azacytidme, angiotensm n, bone morphogenetic protein 2, and pifithrin-α, bone marrow mesenchymal stem cells differentiated towards cardiomyocyte-like cells and expressed myocardium-specific protein Different inductors exert different effects on differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-llike cells which occurs because of different pathways.关键词
骨髓间充质干细胞/诱导剂/诱导/分化/心肌样细胞分类
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刘博武,吕安林,黄炜,侯婧,侯兆蕾,侯红,达晶,杨娜..四种诱导剂诱导骨髓间充质干细胞向心肌样细胞的分化[J].中国组织工程研究,2012,16(14):2471-2476,6.
