中国组织工程研究2012，Vol.16Issue(14)：2487-2490,4.DOI:10.3969/j.issn.1673-8225.2012.14.004
大鼠骨髓间充质干细胞的PKH26标记和示踪
Rat bone marrow mesenchymal stem cells labeled and tracked with PKH26
彭艳 1何援利 1朱少芳1
作者信息
- 1. 南方医科大学珠江医院妇产科,广东省广州市,510282
- 折叠
摘要
Abstract
BACKGROUND: Labeling of bone marrow mesenchymal stem cells (BMSCs) are important links to the research of differentiation and migration in vivo.OBJECTIVE: To label the BMSCs with PKH26 and to explore the effect of PKH26 labeling on the biological activity,differentiation in vifro and tracking in vivo.METHODS: BMSCs were isolated and cultivated from the bone marrow of rats The passage 2 BMSCs were cultured and labeled with PKH26 The proliferation, cycle and apoptotic of cells in labeled group and unlabeled group were evaluated The BMSCs in labeled group were performed with osteoblasts and lipoblasts induction in vitro The PKH26-labeled BMSCs were transplanted through vein and the distribution of BMSCs in rat endometnum was observed under fluorescence microscope at 6 weeks after transplantationRESULTS AND CONCLUSION: The influence of PKH26-labelled cells on cell proliferation, apoptotic and cycle was not significant And the labeled cells still had biological characteristics of osteoblasts and hpoblasts In endometrium, PKH26-labelled cells were mainly distributed in epithelial cells and stromal cells It suggested that the PKH26 labeling method could be used to study the homing, plasticity and transplantation of BMSCs关键词
细胞标记/PKH26/骨髓间充质干细胞/移植/迁移转归分类
医药卫生引用本文复制引用
彭艳,何援利,朱少芳..大鼠骨髓间充质干细胞的PKH26标记和示踪[J].中国组织工程研究,2012,16(14):2487-2490,4.
