中国组织工程研究2012，Vol.16Issue(14)：2520-2523,4.DOI:10.3969/j.issn.1673-8225.2012.14.010
三种大鼠骨髓间充质干细胞双向电泳样品制备方法的比较
Three kinds of two-dimensional gel electrophoresis sample preparation methods of rabbit bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND: A variety of "standard" sample preparation methods for two-dimensional gel electrophoresis have been reported, but there is no single one can be universally applied to rabbit bone marrow mesenchymal stem cells (rBMSCs) OBJECTIVE: To identif a suitable sample preparation method for rBMSCs two-dimensional gel electrophoresis (2-DE) METHODS: After full protein extraction of rBMSCs cultured in vitro, protein samples were divided into three groups untreated, acetone precipitation and 2-D clean-up kit group, the protein concentration was measured by Bradford method, electrophoresis maps were analyzed.RESULTS AND CONCLUSION: Using 2-D clean-up kit to treat the protein sample could obtain the best electrophoresis map which showed lowest background, clearest protein spots, highest resolution and good reproducibility Protein extraction treated with 2-D Clean-up was a more suitable 2-DE sample preparation method of rBMSCs.关键词
双向电泳/骨髓间充质干细胞/大鼠/蛋白质组学/样品制备分类
医药卫生引用本文复制引用
覃万安,苏伟,黄钊,崔向荣,郭杰..三种大鼠骨髓间充质干细胞双向电泳样品制备方法的比较[J].中国组织工程研究,2012,16(14):2520-2523,4.基金项目
国家自然科学基金资助项目(30860078) （30860078）
广西壮族自治区教育厅科研项目(200710MS021)及广西医科大学第一附属医院科研基金项目. （200710MS021）
