中国组织工程研究2012，Vol.16Issue(14)：2530-2533,4.DOI:10.3969/j.issn.1673-8225.2012.14.012
自体脂肪源性干细胞与成纤维细胞联合尿道周围注射治疗大鼠压力性尿失禁
Periurinary tract transplantation of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence
关立铭 1李秀娟 1陈亚萍 1李倩 1吴芸 1陈伟1
作者信息
- 1. 上海第五人民医院妇产科,上海市200240
- 折叠
摘要
Abstract
BACKGROUND: Stem cells proliferated and differented to form fibroblasts and well-suited connective tissue, followed by tissue regeneration, which is a gradually increasing research area for treatment of pelvic organ prolapse and stress urinary incontinence. OBJECTIVE: To investigate the feasibility of periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence.METHODS: Rats were induced to develop stress urinary transplantation by postpartum vaginal balloon dilation and bilateral ovariectomy. One month later, autologous adipose-derived stem cells and fibroblasts were injected into the region surrounding the urinary tract. Another month later, urinary voiding function was assessed by conscious cystometry. At the same time, urinary tract proximal end tissue was stained by hematoxylin-eosin and elastic fibers were stained to observe morphological change. RESULTS AND CONCLUSION: Compared with un-treated rats, rats receiving stem cell treatment exhibited increased leak point pressure (P < 0.01) with normal voiding, thickened urinary tract muscular layer (P < 0.01) and increased elastic fiber and smooth muscle content (P < 0.01). These findings suggest that periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts can be used for treatment of stress urinary incontinence because it can significantly increase abdominal leak point pressure and strengthen urinary tract muscular layer pressure.关键词
脂肪源性干细胞/成纤维细胞/压力性尿失禁/弹力纤维/平滑肌分类
医药卫生引用本文复制引用
关立铭,李秀娟,陈亚萍,李倩,吴芸,陈伟..自体脂肪源性干细胞与成纤维细胞联合尿道周围注射治疗大鼠压力性尿失禁[J].中国组织工程研究,2012,16(14):2530-2533,4.
