中国组织工程研究2012，Vol.16Issue(14)：2559-2562,4.DOI:10.3969/j.issn.1673-8225.2012.14.018
绵羊羊膜上皮细胞分离培养及鉴定
Isolation, culture and identitication of Ovis aries amnion epithelial cells
摘要
Abstract
BACKGROUND: The amnion epithelial cells (AECs) with stem cells properties have been successfully obtained after isolating,digesting and culturing human and rat amnion and AECs have the potential to differentiate into cells of the endoderm (liver, lung,epithelium), mesoderm (bone, fat), and ectoderm (neural cells),OBJECTIVE: To obtain the Ovis aries AECs and detect the stem cell characteristics.METHODS: Ovis aries amnion tissue was peeled off by mechanical way and the low velocity-trypLE (pancreatic enzymereplacement) digestion method was used to isolate and culture the Ovis aries amnion, and the Ovis aries AECs were obtained.RESULTS AND CONCLUSION: The immunofluorescence observation showed that the labeled protein of AECs such as Oct-4,SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 could be expressed on Ovis aries AECs. RT-PCR experiments confirmed thatthe genes of Oct-4, Sox-2 and Rex-1 were expressed and Nanog was not expressed. The results indicate that the Ovis ariesAECs with stem cells properties have been achieved successfully.关键词
绵羊羊膜上皮细胞/分离/培养/细胞免疫荧光/RT-PCR分类
医药卫生引用本文复制引用
朱雪敏,李海军,米焱,杨银凤,关洪敏,曹贵方..绵羊羊膜上皮细胞分离培养及鉴定[J].中国组织工程研究,2012,16(14):2559-2562,4.基金项目
国家高技术研究发展计划(863 计划)项目(2008AA101005). （863 计划）
