中国组织工程研究2012，Vol.16Issue(14)：2563-2566,4.DOI:10.3969/j.issn.1673-8225.2012.14.019
胶原酶和胰酶对胎鼠端脑神经干细胞培养的影响
Effects of trypsogen and collagenase on culture of neural stem cells of fetal rat telencephalon
摘要
Abstract
BACKGROUND: Neural stem cells (NSCs) bring wide prospect for the study of neural development and repairing, and the in vitroculture is foundational to neuroscience.OBJECTIVE: To contrast the effects of trypsogen and collagenase on culture of NSCs in vitro.METHODS: The fetal rat telencephalons were isolated from SD rats pregnant for 14 days under sterile conditions, and tissueswere divisively treated with trypsogen and type I collagenase contained EDTA following trituration, and cultured with serum-freemedium.RESULTS AND CONCLUSION: The adherent monoculture NSCs were obtained using collagenase and congeries of cells wereobtained using trypsogen. Nestin immunofluorescence identification showed the cells were positive, and the purity was over 99%.The primary adherent monoculture NSCs can be simply and quickly got using collagenase.关键词
胰酶/胶原酶/神经干细胞/细胞培养/鉴定/胎鼠分类
医药卫生引用本文复制引用
梁军,宋晓玉,赵富生,李月珍,董建将..胶原酶和胰酶对胎鼠端脑神经干细胞培养的影响[J].中国组织工程研究,2012,16(14):2563-2566,4.基金项目
黑龙江省普通高等学校青年骨干支持项目(1155G60). （1155G60）
