中国组织工程研究2012，Vol.16Issue(14)：2567-2570,4.DOI:10.3969/j.issn.1673-8225.2012.14.020
脐血干细胞移植对帕金森病大鼠旋转行为的影响
Effect of human umbilical cord blood mesenchymal stem cells transplantation on rotational behavior of Parkinson's disease rats
樊志刚 1刘芳1
作者信息
- 1. 河南职工医学院,河南省郑州市451191
- 折叠
摘要
Abstract
BACKGROUND: To date, the clinical treatment of Parkinson's disease (PD) mainly depends on drug, and as for celltransplantation experiment, bone marrow mesenchymal stem cells (BMSCs) transplantation is the common method. The reportsabout whether umbilical cord blood mesenchymal stem cells (UCBMSCs) transplantation can improve the rotational behavior arerare.OBJECTIVE: To explore the effect of human UCBMSCs transplantation on rotational behavior of PD rats.METHODS: The PD rat models were divided into the experimental group (n=20) and the control group (n=20). The fourthgeneration of MSCs were marked by Hoechst33258 and then transplanted into rat striatum in experimental group, and the rats incontrol group were given PBS. Apomorphine was injected intraperitoneally to examine the rotational behavior of the rats in twogroups every week. Immunofluorescence double labeling method was used to identify the survival and migration of MSCs and theexpression of glial fibrillary acidic protein (GFAP), neuron specific enolase (NSE), tyrosine hydroxylase (TH) and synaptophysinat the 3rd, 6th and 9th weeks.RESULTS AND CONCLUSION: The rotational behavior of PD rats in experimetal group was improved obviously compared withcontrol group (P < 0.05) after UCBMSCs transplantation. Part of MSCs could survive in brain tissue, and migrated to the striatum,callose and cortex with time prolonged and expressed GFAP, NSE and TH, but not expressed synaptophysin. UCBMSCs couldobviously improve the rotational behavior of PD rats and were expected to be the seed cells to cure PD.关键词
脐血干细胞/移植/帕金森病/大鼠/旋转行为分类
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樊志刚,刘芳..脐血干细胞移植对帕金森病大鼠旋转行为的影响[J].中国组织工程研究,2012,16(14):2567-2570,4.
