中国组织工程研究2012，Vol.16Issue(14)：2571-2575,5.DOI:10.3969/j.issn.1673-8225.2012.14.021
不同体外环境下舌癌干细胞标志CD44及ESA和CXCR4的表达
Expression of tongue cancer stem cells marker CD44, ESA and CXCR4 in different environments
摘要
Abstract
BACKGROUND: Cancer stem cells marker expression in different environments differ greatly because the microenvironment can impact the expression of proteins.OBJECTIVE: To discuss whether different cultures in vitro have influence on the expressions of cancer stem cells marker CD44, ESA and CXCR4.METHODS: Tongue cancer stem cells TCA8113 cell line was obtained. In vitro, three different microenvironments were established: stem cell culture medium, conventional standard medium and conventional standard medium supplemented with adriamycin. The expressions of three proteins in TCA8113 tongue carcinoma cells were detected by immunohistochemistry (IHC) and flow cytometry (FCM).RESULTS AND CONCLUSION: In all microenvironments, IHC showed the expressions of CD44 and ESA were"++". In stem cell culture medium and the other microenvironments, the expressions of CXCR4 were "±" and "+" respectively. FCM found the expressions of CD44 and ESA were above 90% in all microenvironments; compared with conventional standard medium, the expression of CXCR4 was super low in stem cell culture medium, and recovery when tongue cancer cells reversed into conventional standard medium from stem cell culture medium; the expression of CXCR4 was increased in conventional standard medium supplemented with adriamycin. The expressions of cancer stem cell markers were various in different mircoenvironments; it needs to take various markers into account and combine with the experessions of markers on cancer cells in vivo to identify cancer stem cell markers. Different kinds of cancer stem cells may accumulate together by different microenvironments model.关键词
舌癌/癌干细胞/肿瘤干细胞/体外培养/微环境/TCA8113分类
医药卫生引用本文复制引用
黄英华,姚金光,邝晓聪,蔡捷,解继胜,李俊,陈海波,杨永荣..不同体外环境下舌癌干细胞标志CD44及ESA和CXCR4的表达[J].中国组织工程研究,2012,16(14):2571-2575,5.基金项目
国家自然科学基金(30860326)资助,课题名称:SELDI-TOF -MS 技术筛选舌癌干细胞蛋白标记物的研究. （30860326）
