中国组织工程研究2012，Vol.16Issue(14)：2648-2651,4.DOI:10.3969/j.issn.1673-8225.2012.14.037
急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞与白细胞介素2、白细胞介素10、转化生长因子β的检测
Detection of interleukin 2, interleukin 10, transforming growth factor β and CD4+CD25+ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia
摘要
Abstract
BACKGROUND: At present, the cause of acute lymphoblastic leukemia (ALL) has not yet entirely understood, CD4+CD25+regulatory T cells (Treg) may have certain regulatory effect on immune response in pathogenic process of ALL.OBJECTIVE: To study the levels of interleukin 2 (IL-2), interleukin 10 (IL-10), transforming growth factor (TGF-β) of CD4+CD25+regulatory T cells (Treg) in peripheral blood of patients with ALL.METHODS: The peripheral blood mononuclear cells (PBMC) were isolated with density gradient centrifugation from 22 B cellseries ALL (B-ALL) patients, 13 T cell series ALL (T-ALL) patients and 18 normal people. Immunomagnetic bead separation wasused to separate CD4+CD25+Treg cells, and the levels of IL-2, IL-10 and TGF-β in the cells culture supernatant were detected byusing enzyme-linked immunosorbent assay (ELISA).RESULTS AND CONCLUSION: The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALLpatients were significantly higher than those in normal people, while the level of IL-2 was significantly lower than that in normalpeople (P < 0.05). There was no significant difference between B-ALL and T-ALL patients in the IL-2, IL-10 and TGF-β levels of thecells culture supernatant (P > 0.05). CD4+CD25+Treg cells might interfere the immunologic reaction of antitumor by increasing theIL-10 and TGF-β levels and decreasing the IL-2 level.关键词
急性淋巴细胞白血病/CD4+CD25+T调节细胞/白细胞介素2/白细胞介素10/转化生长因子β分类
医药卫生引用本文复制引用
巫翠萍,覃西,巫翠云,朱洪,周海燕..急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞与白细胞介素2、白细胞介素10、转化生长因子β的检测[J].中国组织工程研究,2012,16(14):2648-2651,4.基金项目
海南省卫生厅科研立项课题(琼卫2008-55). （琼卫2008-55）
