中国组织工程研究2012，Vol.16Issue(15)：2669-2673,5.DOI:10.3969/j.issn.1673-8225.2012.15.003
透骨消痛胶囊干预膝骨性关节炎软骨下骨重塑的分子机制
Molecular mechanism of Tougu Xiaotong capsules for subchondral bone remodeling in knee Osteoarthritis
摘要
Abstract
BACKGROUND: Former study indicates that Tougu Xiaotong capsule is an effective treatment for knee osteoarthritis; it has a protective effect on knee articular cartilage. Subchondral bone remodeling is important in the pathology process of knee osteoarthritis. OBJECTIVE: To observe the effect of Tougu Xiaotong capsule on subchondral bone remodeling in osteoarthritis and to investigate the mechanism. METHODS: New Zealand rabbits were divided into four groups: normal group, model group, Zhuanggu Guanjie pill group and Tougu Xiaotong capsule group. Model construction was performed using modified Hulth method in rabbits except for the normal group. Rabbits in the normal group and model group were administrated with normal saline daily by gavage. Rabbits in the Zhuanggu Guanjie pill group were administrated with the water solution of Zhuanggu Guanjie pill by gavage daily. Rabbits in Tougu Xiaotong capsule group were administrated the water solution of Tougu Xiaotong capsule by gavage daily. RESULTS AND CONCLUSION: The expression of Cyclin D1 gene, insulin-like growth factor and receptor activator of nuclear factor-κB ligand mRNA in the subchondral bone of the Tougu Xiaotong capsule group was significantly higher than that of the normal group in 6, 9 and 12 weeks after mo del construction (P < 0.05). Compared with the model group, the mRNA expression of Cyclin D1 in the Tougu Xiaotong capsule group was lower in 1 week after model construction (P < 0.05); the mRNA expression of insulin-like growth factor and receptor activator of nuclear factor-κB ligand was lower in 6 week after model construction (P < 0.05); the expression of indicators in the Tougu Xiaotong capsule group was relatively lower in 9 and 12 week after model construction (P < 0.05). Compared with Zhuanggu Guanjie pill group, the expression of Cyclin D1 mRNA in the Tougu Xiaotong capsule group was lower in 1 week after model construction (P < 0.05); the expression of receptor activator of nuclear factor-κB ligand in the Tougu Xiaotong capsule group was lower in 6 week after model construction (P < 0.05); the mRNA expression of insulin-like growth factor in the Tougu Xiaotong capsule group was lower in 9 and 12 weeks (P < 0.05). These findings indicate that Tougu Xiaotong capsule can change the rates and patterns of subchondral bone remodeling, and ultimately reduce the subchondral bone sclerosis.关键词
透骨消痛胶囊/骨性关节炎/软骨下骨/Cyclin D1/胰岛素样生长因子/分子生物学分类
医药卫生引用本文复制引用
李民,郭逸尔,刘献祥,魏双胜,陈文列,林久茂,黄云梅,黄梅雅,吴追乐..透骨消痛胶囊干预膝骨性关节炎软骨下骨重塑的分子机制[J].中国组织工程研究,2012,16(15):2669-2673,5.基金项目
福建省自然科学基金(2010J01368)、福建省漳州市中医院院内课题(ZZZYY2010-04). （2010J01368）
