中国组织工程研究2012，Vol.16Issue(15)：2681-2685,5.DOI:10.3969/j.issn.1673-8225.2012.15.005
miR-3960对成骨细胞分化的影响
Effect of miR-3960 on osteogenic differentiation
摘要
Abstract
BACKGROUND: Osteoblast differentiation involves highly regulated processes mediated by a large number of hormones and transcription factors. Emerging evidence reveals an additional level of regulation that is mediated by microRNAs. miR-3960 is preferentially expressed in osteoblasts, which suggests that miR-3960 may take part in the regulation process of osteogenic differentiation. OBJECTIVE: To investigate the role of miR-3960 in osteogenic differentiation of primary mouse bone marrow stromal cells. METHODS: The parameters of osteoblast differentiation were measured after transfection of bone marrow stromal cells with pSilencer4.1-miR-3960. Osteoblastic differentiation was induced by the addition of 300 μg/L bone morphogenetic protein 2. RESULTS AND CONCLUSION: Stable and high expression of miR-3960 was obtained in the cells after transfection with pSilencer4.1-miR-3960. Compared with control cells, the levels of alkaline phosphatase activity, osteocalcin secretion and calcium deposition were increased by transfection of pSilencer4.1-miR-3960. Treatment with anti-miR-3960 attenuated the levels of alkaline phosphatase activity and osteocalcin secretion as well as reduced calcium deposition. All these results suggest that miR-3960 promotes osteoblast differentiation of bone marrow stromal cells.关键词
微小RNA/骨髓基质细胞/成骨细胞/分化/过表达/小鼠/转染分类
医药卫生引用本文复制引用
李辉,庞雅玲,李晓燕,王欣文,吴贵福,郭剑,罗湘杭..miR-3960对成骨细胞分化的影响[J].中国组织工程研究,2012,16(15):2681-2685,5.基金项目
本课题受陕西省自然科学基金青年项目(2011JQ4006)及国家青年科学基金(81100208)资助. （2011JQ4006）
