中国组织工程研究2012，Vol.16Issue(15)：2709-2714,6.DOI:10.3969/j.issn.1673-8225.2012.15.011
热休克蛋白90和凋亡抑制蛋白Livin在病理性瘢痕中的表达
Expression of heat shock protein 90 and inhibitor of apoptosis protein Livin in pathologic scars
王大伟 1王喜梅 2刘林嶓 1王琪影 1彭倩 1张娟 1孙玉峰 1张琼阁2
作者信息
- 1. 郑州大学第一附属医院整形外科,河南省郑州市,450052
- 2. 河南省高等学校临床医学重点学科开放实验室,河南省郑州市,450052
- 折叠
摘要
Abstract
BACKGROUND: In recent years, related research has shown that heat shock protein 90 (HSP90) and inhibitor of apoptosis protein Livin play a very important role in the process of cell apoptosis. However, the roles of HSP90 and Livin in the formation of pathologic scars are rarely reported. OBJECTIVE: To investigate the expression and correlation of HSP90 and Livin in pathologic scars in gene and protein levels. METHODS: Reverse transcription-PCR and SP immunohistochemical methods were used to detect the expressions of HSP90 and Livin in 24 cases of keloid, 26 cases of hypertrophic scar, 22 cases of non-pathologic scar and 20 cases of normal skin tissue. RESULTS AND CONCLUSION: The positive levels of HSP90 and Livin in the keloid group and hypertrophic scar group were higher than that in the non-pathologic scar group and normal skin group (P < 0.05). There was a positive correlation between HSP90 and Livin expressions in the pathologic scars (rs=0.436, P < 0.05). RT-PCR results were similar to the immunohistochemical results. The results suggest that HSP90 and Livin may play an important role in the formation of pathologic scar; and there may be a synergistic effect between the two.关键词
热休克蛋白90/Livin/病理性瘢痕/反转录聚合酶链式反应/免疫组织化学/凋亡分类
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王大伟,王喜梅,刘林嶓,王琪影,彭倩,张娟,孙玉峰,张琼阁..热休克蛋白90和凋亡抑制蛋白Livin在病理性瘢痕中的表达[J].中国组织工程研究,2012,16(15):2709-2714,6.
