中国组织工程研究2012，Vol.16Issue(15)：2720-2723,4.DOI:10.3969/j.issn.1673-8225.2012.15.013
三七总皂苷对L6大鼠成肌细胞抗化学损伤模型的保护作用
Protective effect of panax notoginseng saponin against chemical damage of myoblasts in L6 rats
摘要
Abstract
BACKGROUND: Panax notoginseng saponin has multiple pharmacological effects such as stasis and clearing and activating the channels and collaterals, but the mechanisms are unclear. OBJECTIVE: To observe the protective effect of panax notoginseng saponin against H2O2 damage of L6 rat myoblasts and its effect on the expression of Bax and bcl-2. METHODS: H2O2 damaged model of L6 rat myoblasts was built. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 4,6-diamino-2-phenyl indole chemical fluorescence and flow cytometry were applied to detect the survival rates of L6 rat myoblasts treated with different dosesof panax notoginseng saponin (0.5 , 0.1 and 0.02 g/L). The effects of different doses of panax notoginseng saponin (0.5, 0.1 and 0.02 g/L) on Bax and Bcl-2 protein expression levels of L6 rat myoblasts were analyzed by immunofluorescence cytochemistry. RESULTS AND CONCLUSION: The L6 rat myoblasts had low survival rate and high apoptosis rate after injury of H2O2, while panax notoginseng saponin could obviously increase the survival rate and reduce the apoptosis rate. Immunofluorescence cytochemistry results showed that panax notoginseng saponin could enhance the Bcl-2 expression and inhibit the Bax expression. Panax notoginseng saponin can protect L6 rat myoblasts from H2O2 damage, and the protective effects are in order of sequence of 0.1 g/L > 0.02 g/L > 0.5 g/L . The mechanism may be related to the enhancement of Bcl-2 expression and inhibition of Bax expression.关键词
三七总皂苷/L6/大鼠/成肌细胞/过氧化氢/Bcl-2/Bax分类
医药卫生引用本文复制引用
杨燕华,朱道立,陈佩林,余春梅,杨玉芹..三七总皂苷对L6大鼠成肌细胞抗化学损伤模型的保护作用[J].中国组织工程研究,2012,16(15):2720-2723,4.基金项目
南通大学大学生创新训练计划资助项目(2010038),南通大学博士点建设专项(05024276). （2010038）
