中国组织工程研究2012，Vol.16Issue(15)：2733-2736,4.DOI:10.3969/j.issn.1673-8225.2012.15.016
电穿孔介导基因治疗下颌骨牵引过程中血管内皮细胞生长因子的表达
Effects of electroporation-mediated gene therapy on the expression of vascular endothelial growth factor during mandible distraction
摘要
Abstract
BACKGROUND: Our previous studies have proved the gene therapy can promote new bone formation in mandibular distraction area. OBJECTIVE: To investigate the effect of electroporation-mediated gene therapy on the expression of vascular endothelial growth factor (VEGF) in the distracted calluses during mandibular distraction osteogenesis in a rabbit model. METHODS: Bilateral mandibular osteotomies were performed in 50 New-Zeland rabbits. The mandibular distraction osteogenesis models were established. After the completion of distraction, the rabbits were randomly divided into five groups: group A: pIRES-hVEGF165-hBMP2; group B: pIRES-hBMP2; group C: pIRES-hVEGF165; group D: pIRES and group E: normal saline. All the animals were treated with electroporation stimulation. RESULTS AND CONCLUSION: VEGF staining was located in cytoplasm of endothelial cells, osteoblasts, fibroblasts, inflammatory cells and immature osteocytes. Their strongest expression was found at 7 days after the end of distraction, declined at 14 days, and weakened with maturation of the newly formed bone at 28 days. But in every point, the gene therapy group was stronger than the control group. It suggests that electroporation-mediated gene therapy can obtain continues expression of VEGF, and make the meaning extended time limit, which can promote angiogenesis during mandibular distraction, and new bone formation.关键词
电穿孔/基因疗法/骨生成/牵张/血管内皮细胞生长因子分类
医药卫生引用本文复制引用
尹康,胡纯兵,何小川,李绍兰,吴国平,郭力..电穿孔介导基因治疗下颌骨牵引过程中血管内皮细胞生长因子的表达[J].中国组织工程研究,2012,16(15):2733-2736,4.基金项目
国家自然科学基金资助项目(30600653) （30600653）
四川省应用基础研究计划项目(2006J13-128) （2006J13-128）
四川省卫生厅科研课题(060044,100285). （060044,100285）
